Chymotrypsin from Helicoverpa armigera

Enzyme Description

Species
Helicoverpa armigera β†—
Extremophile
No
EC Number
3.4.21.1 β†—

Sequence

Length: 295 amino acids
MKFVALTLLALAAVASARNVHLEDSIDLEDITAWGYLTKFGIPEAEKIRSAEEASSASRIVGGSLSSLGQIPYQAGLVIDLSGGQAVCGGSLISASRVLTAAHCWFDGQNQAWRFTVVLGSTTLFSGGTRIATSNVVMHGSWTPSLIRNDVAVIRLGTNVGTSNTIAIIALPSGSQINENFAGETALASGFGLTSDSGSISSNQALSHVNLPVITNAVCRSSFPLLIQDSNICTSGANGRSTCRGDSGGPLAVTRNSRPLLIGITSFGSARGCQVGSPAAFARVTSYISWINGQL
Shaik Mohammad Akbar et al. (2016) β€” Alkaline serine proteases from Helicoverpa armigera : potential candidates for industrial applications
Archives of Insect Biochemistry and Physiology  Β· doi:10.1002/arch.21367 β†—  Β· Activity - Classical
12 measurements ▼
Database ID
UniProt: D7RZZ6 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, insect Helicoverpa armigera

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent Methanol 5 % v/v 100.0 114.39 % Direct Continuous 100 mM glycine–NaOH buffer 10 37Β°C 0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-Nitroaniline , Suc-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 5 % v/v 100.0 105.77 % Direct Continuous 100 mM glycine–NaOH buffer 10 37Β°C 0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-Nitroaniline , Suc-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent 1-Propanol 5 % v/v 100.0 97.33 % Direct Continuous 100 mM glycine–NaOH buffer 10 37Β°C 0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-Nitroaniline , Suc-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent 1-Butanol 5 % v/v 100.0 105.3 % Direct Continuous 100 mM glycine–NaOH buffer 10 37Β°C 0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-Nitroaniline , Suc-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent Acetone 5 % v/v 100.0 112.55 % Direct Continuous 100 mM glycine–NaOH buffer 10 37Β°C 0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-Nitroaniline , Suc-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent n-Hexane 5 % v/v 100.0 111.62 % Direct Continuous 100 mM glycine–NaOH buffer 10 37Β°C 0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-Nitroaniline , Suc-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5 % v/v 100.0 118.98 % Direct Continuous 100 mM glycine–NaOH buffer 10 37Β°C 0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-Nitroaniline , Suc-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent Dimethylformamide (DMF) 5 % v/v 100.0 110.17 % Direct Continuous 100 mM glycine–NaOH buffer 10 37Β°C 0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-Nitroaniline , Suc-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent Benzene 5 % v/v 100.0 112.59 % Direct Continuous 100 mM glycine–NaOH buffer 10 37Β°C 0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-Nitroaniline , Suc-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent Toluene 5 % v/v 100.0 108.69 % Direct Continuous 100 mM glycine–NaOH buffer 10 37Β°C 0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-Nitroaniline , Suc-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent Diethyl Ether 5 % v/v 100.0 90.0 % Direct Continuous 100 mM glycine–NaOH buffer 10 37Β°C 0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-Nitroaniline , Suc-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent Chloroform 5 % v/v 100.0 106.06 % Direct Continuous 100 mM glycine–NaOH buffer 10 37Β°C 0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-Nitroaniline , Suc-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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