Esterase (gene Lip2) from Monascus purpureus M7

Enzyme Description

Species
Monascus purpureus M7 β†—
Extremophile
No
EC Number
3.1.1.1 β†—

Sequence

Length: 258 amino acids
MSSIEEGTSASGAQGLLYQPYDQFMLFGDSITEFSCNQSMGFAFHPALQNSYIRRLDVINRGFSGYTSANALQVLPSFFPTPRTAKVRLMTIFFGANDACLPGKFQHVPLGEYKKNLEKLIEHPAVKEQNPRIIIITPPPVNEYQFFDMDDPSRIASNTKLYAEAAREVAGSLGVPVADIWTRFMEAAGWQEGQPLLGSRDVPESEKLQSFLTDGLHLTPDGYRIVYEEVMEVIRVNWPDQTPEQLSRVFPGWQDAPK
Li-Jing Kang et al. (2017) β€” Screening, purification, and characterization of a novel organic solvent-tolerant esterase, Lip2, from Monascus purpureus strain M7
Extremophiles  Β· doi:10.1007/s00792-016-0907-x β†—  Β· Activity - Classical Stability - Incubation
48 measurements ▼
Database ID
UniProt: A0A1B2CT54 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (48 measurements)

48 measurements β€” page 2 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Ethanol 20 % v/v 100 107 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Acetonitrile 20 % v/v 100 99 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 20 % v/v 100 107 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Glycerol 20 % v/v 100 109 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Acetone 20 % v/v 100 102 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase n-Hexane 20 % v/v 100 98 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Toluene 20 % v/v 100 89 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Cyclohexane 20 % v/v 100 72 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase n-Hexane 20 % v/v 100 71 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase n-Heptane 20 % v/v 100 102 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Cyclohexane 20 % v/v 100 98 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Methanol 20 % v/v 100 115 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Ethanol 20 % v/v 100 124 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Acetonitrile 20 % v/v 100 147 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 20 % v/v 100 96 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Glycerol 20 % v/v 100 105 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Acetone 20 % v/v 100 101 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase n-Hexane 20 % v/v 100 104 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Toluene 20 % v/v 100 132 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Acetone 20 % v/v 100 126 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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