Is3-TA amine transaminase from a hot spring metagenomic library

Enzyme Description

Species
Na (hot spring metagenomic library)
Extremophile
Yes thermophilic organism (from a hot spring)
EC Number
2.6.1.18 β†—

Sequence

Length: 466 amino acids
MKPNDLPEFDTAALRAKDAAHHLHPFTDTKSLNAAGSRVIVRGEGVRIWDSDGNEIIDGMSGLWCVNLGYGRQDLVDAATRQMQTLPFYNAFFKTTTAPATELAALLPQVAPPGFNHVFFTNSGSEGNDTIIRLVRRYWDLMGQPQRKTIISRHNAYHGSTLGGASLGGMSGMHEQGDLPLPGITHIDQPYQLQHGKPGESAHDFGLRAASWLEAKILELGADKVAAFIGEPVQGAGGVIIPPASYWPEIQRICDKYGLLLISDEVICGFGRLGHWFGCQHPHINSKPDLITFAKGVTSGYLPLGGVLVGDRVAKVLIEQGGDFNHGFTYSGHPTCCAVAIANVSALRNEGVVERVATDTAPYLKAQFATLADHPLVGDVDSLGMVAGLVLYKDKASRTLFDADLGVGYLCRDHCFKNGVVMRAVEERMIIAPPLVMTHVEIDEMIRRIRRSLDDTLAGLRAQGLV
Erica Elisa Ferrandi et al. (2017) β€” Novel thermostable amine transferases from hot spring metagenomes
Applied Microbiology and Biotechnology  Β· doi:10.1007/s00253-017-8228-2 β†—  Β· Stability - Incubation
12 measurements ▼
Database ID
UniParc: UPI0007C3445A β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli Rosetta

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (5 hours at 25Β°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase Methanol 5 % v/v 39 39 % Digital Continuous Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 0.25% DMSO (Dimethyl Sulfoxide) Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 20Β°C 2.5 mM (S)-1-Phenylethylamine, 2.5 mM Pyruvate Acetophenone , L-Alanine β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (5 hours at 25Β°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase Methanol 10 % v/v 39 47 % Digital Continuous Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 0.25% DMSO (Dimethyl Sulfoxide) Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 20Β°C 2.5 mM (S)-1-Phenylethylamine, 2.5 mM Pyruvate Acetophenone , L-Alanine β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (5 hours at 25Β°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase Methanol 20 % v/v 39 23 % Digital Continuous Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 0.25% DMSO (Dimethyl Sulfoxide) Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 20Β°C 2.5 mM (S)-1-Phenylethylamine, 2.5 mM Pyruvate Acetophenone , L-Alanine β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (5 hours at 25Β°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase Ethanol 5 % v/v 39 39 % Digital Continuous Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 0.25% DMSO (Dimethyl Sulfoxide) Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 20Β°C 2.5 mM (S)-1-Phenylethylamine, 2.5 mM Pyruvate Acetophenone , L-Alanine β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (5 hours at 25Β°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase Ethanol 10 % v/v 39 100 % Digital Continuous Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 0.25% DMSO (Dimethyl Sulfoxide) Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 20Β°C 2.5 mM (S)-1-Phenylethylamine, 2.5 mM Pyruvate Acetophenone , L-Alanine β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (5 hours at 25Β°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase Ethanol 20 % v/v 39 60 % Digital Continuous Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 0.25% DMSO (Dimethyl Sulfoxide) Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 20Β°C 2.5 mM (S)-1-Phenylethylamine, 2.5 mM Pyruvate Acetophenone , L-Alanine β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (5 hours at 25Β°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 5 % v/v 39 100 % Digital Continuous Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 0.25% DMSO (Dimethyl Sulfoxide) Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 20Β°C 2.5 mM (S)-1-Phenylethylamine, 2.5 mM Pyruvate Acetophenone , L-Alanine β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (5 hours at 25Β°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10 % v/v 39 71 % Digital Continuous Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 0.25% DMSO (Dimethyl Sulfoxide) Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 20Β°C 2.5 mM (S)-1-Phenylethylamine, 2.5 mM Pyruvate Acetophenone , L-Alanine β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (5 hours at 25Β°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 20 % v/v 39 57 % Digital Continuous Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 0.25% DMSO (Dimethyl Sulfoxide) Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 20Β°C 2.5 mM (S)-1-Phenylethylamine, 2.5 mM Pyruvate Acetophenone , L-Alanine β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (5 hours at 25Β°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase Acetonitrile 5 % v/v 39 60 % Digital Continuous Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 0.25% DMSO (Dimethyl Sulfoxide) Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 20Β°C 2.5 mM (S)-1-Phenylethylamine, 2.5 mM Pyruvate Acetophenone , L-Alanine β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (5 hours at 25Β°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase Acetonitrile 10 % v/v 39 37 % Digital Continuous Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 0.25% DMSO (Dimethyl Sulfoxide) Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 20Β°C 2.5 mM (S)-1-Phenylethylamine, 2.5 mM Pyruvate Acetophenone , L-Alanine β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (5 hours at 25Β°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase Acetonitrile 20 % v/v 39 0 % Digital Continuous Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 0.25% DMSO (Dimethyl Sulfoxide) Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 20Β°C 2.5 mM (S)-1-Phenylethylamine, 2.5 mM Pyruvate Acetophenone , L-Alanine β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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