Aminopeptidase (gene mesoamp) from Mesorhizobium SEMIA300

Enzyme Description

Species
Mesorhizobium SEMIA300 β†—
Extremophile
No
EC Number
3.4.1.1 β†—

Sequence

Length: 418 amino acids
MTTHSRGVSATIDPVKLDRLAEVAIKVGLQLQPGQDLVLTSSIAALPLTRRIVEHAYKAGAGLVTPIFNDDEMTLARYRFGADASFDRAAGWLYEGMAKAFSNNAARLAVRGEDPSLLSAQDPAKVARANKANSMAYQPALEKITGFDINWNIVAYPDLAWAKQVFPGDADDVAVAKLADAIFAASRVDVEDPIGNWKAHNAALRGRTEWLNGHNFHALHFTGPGTDLTVGLADGHEWMGGASTAKNGITCNPNIPTEEVFTTPHARRVEGHVSSTKPLSYQGTLIDEISVRFEEGRIVEAKASKGEDVLKKVLDTDEGARRLGEVALVPHSSPISASGLLFFNTLFDENAACHIALGQCYSKCFVDGASLSQDEIAARGGNKSFIHIDWMIGSDKIDIDGVHKDGSRVPVMRKGEWA
Elwi Machado Sierra et al. (2017) β€” Halotolerant aminopeptidase M29 from Mesorhizobium SEMIA 3007 with biotechnological potential and its impact on biofilm synthesis
Scientific Reports  Β· doi:10.1038/s41598-017-10932-8 β†—  Β· Stability - Incubation
32 measurements ▼
Database ID
UniParc: UPI00083DE3DB β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (32 measurements)

32 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Cyclohexane 5 % v/v 100 97 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase n-Hexane 40 % v/v 100 30 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase n-Hexane 20 % v/v 100 81 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase n-Hexane 10 % v/v 100 117 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase n-Hexane 5 % v/v 100 109 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase n-Heptane 40 % v/v 100 47 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase n-Heptane 20 % v/v 100 55 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase n-Heptane 10 % v/v 100 75 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase n-Heptane 5 % v/v 100 102 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Chloroform 40 % v/v 100 54 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Chloroform 20 % v/v 100 41 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Chloroform 10 % v/v 100 65 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Chloroform 5 % v/v 100 90 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Cyclohexane 40 % v/v 100 0 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Cyclohexane 20 % v/v 100 0 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Cyclohexane 10 % v/v 100 121 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Acetone 5 % v/v 100 74 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Methanol 40 % v/v 100 40 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Methanol 20 % v/v 100 104 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Methanol 10 % v/v 100 80 % Digital Continuous Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
β€Ή Prev
1 2
Next β€Ί

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate Β· Scroll to zoom Β· Right-click drag to pan Β· Powered by Mol*