Ξ±-amylase from Bacillus licheniformis B4-423

Enzyme Description

Species
Bacillus licheniformis B4-423 β†—
Extremophile
Yes "thermophilic" and "acidophilic" organism (from hot springs)
EC Number
3.2.1.1 β†—

Sequence

Length: 512 amino acids
MKQQKRLYARLLTLLFALIFLLPHSAAAAANLNGTLMQYFEWYMPNDGQHWKRLQNDSAYLAEHGITAVWIPPAYKGTSQADVGYGAYDLYDLGEFHQKGTVRTKYGTKGELQSAIKSLHSRDINVYGDVVINHKGGADATEDVTAVEVDPADRNRVISGEHRIKAWTHFHFPGRGSTYSDFKWHWYHFDGTDWDESRKLNRIYKFQGKAWDWEVSNENGNYDYLMYADIDYDHPDVAAEIKRWGTWYANELQLDGFRLDAVKHIKFSFLRDWVNHVREKTGKEMFTVAEYWQNDLGALENYLNKTNFNHSVFDVPLHYQFHAASTQGGGYDMRKLLNGTVVSKHPLKAVTFVDNHDTQPGQSLESTVQTWFKPLAYAFILTRESGYPQVFYGDMYGTKGDSQREIPALKHKIEPILKARKQYAYGAQHDYFDHHDIVGWTREGDSSVANSGLAALITDGPGGAKRMYVGRQNAGETWHDITGNRSEPVVINSEGWGEFHVNGGSVSIYVQR
Xiangrong Wu et al. (2018) β€” Purification and biochemical characterization of a thermostable and acid-stable alpha-amylase from Bacillus licheniformis B4-423
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2017.12.004 β†—  Β· Stability - Incubation
16 measurements ▼
Database ID
UniProt: L7X1Q1 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Purified, bacterium Bacillus licheniformis

Experimental Data (16 measurements)

16 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Methanol 10 % v/v 100.0 98.8 % Direct Continuous Incubation: 50 mM sodium citrate buffer, Assay: 50 mM sodium citrate buffer Incubation: 5, Assay: 5 Incubation: Room temperature, Assay: 100Β°C 1% (w/v) Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethanol 10 % v/v 100.0 98.0 % Direct Continuous Incubation: 50 mM sodium citrate buffer, Assay: 50 mM sodium citrate buffer Incubation: 5, Assay: 5 Incubation: Room temperature, Assay: 100Β°C 1% (w/v) Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetone 10 % v/v 100.0 103.2 % Direct Continuous Incubation: 50 mM sodium citrate buffer, Assay: 50 mM sodium citrate buffer Incubation: 5, Assay: 5 Incubation: Room temperature, Assay: 100Β°C 1% (w/v) Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isopropanol 10 % v/v 100.0 17.3 % Direct Continuous Incubation: 50 mM sodium citrate buffer, Assay: 50 mM sodium citrate buffer Incubation: 5, Assay: 5 Incubation: Room temperature, Assay: 100Β°C 1% (w/v) Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Butanol 10 % v/v 100.0 11.0 % Direct Continuous Incubation: 50 mM sodium citrate buffer, Assay: 50 mM sodium citrate buffer Incubation: 5, Assay: 5 Incubation: Room temperature, Assay: 100Β°C 1% (w/v) Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isoamyl Alcohol 10 % v/v 100.0 101.4 % Direct Continuous Incubation: 50 mM sodium citrate buffer, Assay: 50 mM sodium citrate buffer Incubation: 5, Assay: 5 Incubation: Room temperature, Assay: 100Β°C 1% (w/v) Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Chloroform 10 % v/v 100.0 98.7 % Direct Continuous Incubation: 50 mM sodium citrate buffer, Assay: 50 mM sodium citrate buffer Incubation: 5, Assay: 5 Incubation: Room temperature, Assay: 100Β°C 1% (w/v) Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase n-Hexane 10 % v/v 100.0 101.6 % Direct Continuous Incubation: 50 mM sodium citrate buffer, Assay: 50 mM sodium citrate buffer Incubation: 5, Assay: 5 Incubation: Room temperature, Assay: 100Β°C 1% (w/v) Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Methanol 20 % v/v 100.0 96.1 % Direct Continuous Incubation: 50 mM sodium citrate buffer, Assay: 50 mM sodium citrate buffer Incubation: 5, Assay: 5 Incubation: Room temperature, Assay: 100Β°C 1% (w/v) Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethanol 20 % v/v 100.0 99.5 % Direct Continuous Incubation: 50 mM sodium citrate buffer, Assay: 50 mM sodium citrate buffer Incubation: 5, Assay: 5 Incubation: Room temperature, Assay: 100Β°C 1% (w/v) Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetone 20 % v/v 100.0 85.1 % Direct Continuous Incubation: 50 mM sodium citrate buffer, Assay: 50 mM sodium citrate buffer Incubation: 5, Assay: 5 Incubation: Room temperature, Assay: 100Β°C 1% (w/v) Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isopropanol 20 % v/v 100.0 14.2 % Direct Continuous Incubation: 50 mM sodium citrate buffer, Assay: 50 mM sodium citrate buffer Incubation: 5, Assay: 5 Incubation: Room temperature, Assay: 100Β°C 1% (w/v) Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Butanol 20 % v/v 100.0 12.7 % Direct Continuous Incubation: 50 mM sodium citrate buffer, Assay: 50 mM sodium citrate buffer Incubation: 5, Assay: 5 Incubation: Room temperature, Assay: 100Β°C 1% (w/v) Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isoamyl Alcohol 20 % v/v 100.0 94.6 % Direct Continuous Incubation: 50 mM sodium citrate buffer, Assay: 50 mM sodium citrate buffer Incubation: 5, Assay: 5 Incubation: Room temperature, Assay: 100Β°C 1% (w/v) Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Chloroform 20 % v/v 100.0 98.2 % Direct Continuous Incubation: 50 mM sodium citrate buffer, Assay: 50 mM sodium citrate buffer Incubation: 5, Assay: 5 Incubation: Room temperature, Assay: 100Β°C 1% (w/v) Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase n-Hexane 20 % v/v 100.0 97.7 % Direct Continuous Incubation: 50 mM sodium citrate buffer, Assay: 50 mM sodium citrate buffer Incubation: 5, Assay: 5 Incubation: Room temperature, Assay: 100Β°C 1% (w/v) Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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