Lipase ZC12 from Psychrobacter sp. ZY124

Enzyme Description

Species
Psychrobacter sp. ZY124 β†—
Extremophile
Yes "psychrophilic" organism
EC Number
3.1.1.3 β†—

Sequence

Length: 358 amino acids
MTLSLYIRAALTPKTLLPILGSISAGVLLMTIQTTSAQAAGQYYNCANANGCKLVDSKYFTSSYTKTQYPVVLAHGLGGFTTLFGVIDYFNGIPEDLMKGGSEVYTTKTSAVNNSEVRGEQLLQQVKTIAAISGESKVNLFGHSQGGIDIRYVAGVAPKYVASVTAVSSPEQGSKTADFVKNVLEPNNTSGQPSNVTTQLVSGVFNLIGGFTDIGSGISFNQIQQQDGWQALVALSTDGAAKFNAKFPAAMPKNYCGQPTSTAVNGIKYYSFSGVGQITSALDPSDYLLAATGVPFAGESNDGLVSACSSRLGYVIRDNYRMNHLDSADQVLGLTAWGESEPKSIYRTQVNRLKNANL
Yue Zhang et al. (2018) β€” Purification and characterization of a novel organic solvent-tolerant and cold-adapted lipase from Psychrobacter sp. ZY124
Extremophiles  Β· doi:10.1007/s00792-018-0997-8 β†—  Β· Activity - Classical Activity + Stability - Incubation
72 measurements ▼
Database ID
UniParc: UPI00080982BE β†—
Sequence Annotation
Explicit - Provided
Protein Source
Purified, cold-adapted bacterium Psychrobacter sp. ZY124

Experimental Data (72 measurements)

72 measurements β€” page 2 of 4
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent n-Octane 50 % v/v 100 140 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent n-Octane 70 % v/v 100 184 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent n-Octane 90 % v/v 100 96 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetonitrile 30 % v/v 100 80 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetonitrile 50 % v/v 100 89 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100 80 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 50 % v/v 100 63 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 70 % v/v 100 56 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 90 % v/v 100 25 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 10 % v/v 100 89 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 30 % v/v 100 91 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 50 % v/v 100 81 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 70 % v/v 100 85 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 90 % v/v 100 42 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 10 % v/v 100 90 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 50 % v/v 100 88 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 70 % v/v 100 64 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 90 % v/v 100 49 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetone 10 % v/v 100 91 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetone 30 % v/v 100 93 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, ?% isopropanol, 1% Triton X-100, 0.25g/100 mL gum arabic Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 37Β°C 10 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 200 rpm Classical aqueous control (in %), assay in the presence of organic solvent | Assay assay over a relatively long period (5 hours)
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Visualization : Activity β€” Classical

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Visualization : Activity + Stability β€” Incubation

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Structure

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