Laccase (gene CtLac) from Caldalkalibacillus thermarum TA2.A1

Enzyme Description

Species
Caldalkalibacillus thermarum strain TA2.A1 β†—
Extremophile
Yes "thermoalkaliphilic" organism
EC Number
1.10.3.2 β†—

Sequence

Length: 516 amino acids
MKRILTLVLLLTVFIIVLVGCSTSPVDHSKMVHGEDDSTDNKQQEETQQIETSEGKVVINLEAKVTHWMFNNDLMEDIWTYNGSLPGQEIRVQEGDHVVINLTNSLDVPTSLHLHGVPVPNEMDGVPGVTQNAIMPGETFTYEFVADTPGTYWYHSHQDGANQIGMGLYGALVVEHKDQPDYDLDQVIMIDEWSSMGMADMDHSGMDHGDHGNMGDNSDQPPHAEMMNMMYDTLIVNGKASPVIEAIGVSEDDTIKLRFVNTGLFTQVITIPGHEFKVTHYDGQQVNEPELISDTAIRIAPAERYDVEIEMNQPGAWGIQIYAEENENLRGIVPLVYAGFEDEELQTADSIQSFLDISAYGSPMNVNFGEITKEFDMILGSDDGGETFTINGKQMTTKGFESHEDHEIYEVEEGDVVKVNIVNDTDVDHPMHLHGHFFYVVSRNGQPVKGSPILKETLNVRPNEAYEIVFVADNPGHWMFHCHELHHASGGMVSEVRYSGYTPAFTPDPNIPNKPE
Sunil Ghatge et al. (2018) β€” A novel laccase from thermoalkaliphilic bacterium Caldalkalibacillus thermarum strain TA2.A1 able to catalyze dimerization of a lignin model compound
Applied Microbiology and Biotechnology  Β· doi:10.1007/s00253-018-8898-4 β†—  Β· Activity - Classical
15 measurements ▼
Database ID
UniParc: UPI00020F0D7F β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (15 measurements)

15 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical absorbance measurement, 420 nm) in the presence of organic solvent Acetone 10 % v/v 100 117 % Direct Continuous 50 mM citrate-phosphate 8 37Β°C 0.5 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 1 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical absorbance measurement, 420 nm) in the presence of organic solvent Acetone 30 % v/v 100 34 % Direct Continuous 50 mM citrate-phosphate 8 37Β°C 0.5 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 1 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical absorbance measurement, 420 nm) in the presence of organic solvent Acetone 50 % v/v 100 0 % Direct Continuous 50 mM citrate-phosphate 8 37Β°C 0.5 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 1 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical absorbance measurement, 420 nm) in the presence of organic solvent Acetonitrile 10 % v/v 100 101 % Direct Continuous 50 mM citrate-phosphate 8 37Β°C 0.5 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 1 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical absorbance measurement, 420 nm) in the presence of organic solvent Acetonitrile 30 % v/v 100 71 % Direct Continuous 50 mM citrate-phosphate 8 37Β°C 0.5 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 1 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical absorbance measurement, 420 nm) in the presence of organic solvent Acetonitrile 50 % v/v 100 32 % Direct Continuous 50 mM citrate-phosphate 8 37Β°C 0.5 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 1 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical absorbance measurement, 420 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100 131 % Direct Continuous 50 mM citrate-phosphate 8 37Β°C 0.5 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 1 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical absorbance measurement, 420 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30 % v/v 100 80 % Direct Continuous 50 mM citrate-phosphate 8 37Β°C 0.5 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 1 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical absorbance measurement, 420 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 50 % v/v 100 23 % Direct Continuous 50 mM citrate-phosphate 8 37Β°C 0.5 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 1 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical absorbance measurement, 420 nm) in the presence of organic solvent Ethanol 10 % v/v 100 152 % Direct Continuous 50 mM citrate-phosphate 8 37Β°C 0.5 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 1 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical absorbance measurement, 420 nm) in the presence of organic solvent Ethanol 30 % v/v 100 84 % Direct Continuous 50 mM citrate-phosphate 8 37Β°C 0.5 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 1 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical absorbance measurement, 420 nm) in the presence of organic solvent Ethanol 50 % v/v 100 20 % Direct Continuous 50 mM citrate-phosphate 8 37Β°C 0.5 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 1 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical absorbance measurement, 420 nm) in the presence of organic solvent Methanol 10 % v/v 100 140 % Direct Continuous 50 mM citrate-phosphate 8 37Β°C 0.5 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 1 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical absorbance measurement, 420 nm) in the presence of organic solvent Methanol 30 % v/v 100 83 % Direct Continuous 50 mM citrate-phosphate 8 37Β°C 0.5 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 1 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical absorbance measurement, 420 nm) in the presence of organic solvent Methanol 50 % v/v 100 39 % Direct Continuous 50 mM citrate-phosphate 8 37Β°C 0.5 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 1 mM CuCl2 β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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