Transglutaminase from Streptomyces mobaraensis

Enzyme Description

Species

Extremophile

EC Number

2.3.2.13 ↗

Sequence

Length: 339 amino acids

DSDDRVTPPAEPLDRMPDPYRPSYGRAETVVNNYIRKWQQVYSHRDGRKQQMTEEQREWLSYGCVGVTWVNSGQYPTNRLAFASFDEDRFKNELKNGRPRSGETRAEFEGRVAKESFDEEKGFQRAREVASVMNRALENAHDESAYLDNLKKELANGNDALRNEDARSPFYSALRNTPSFKERNGGNHDPSRMKAVIYSKHFWSGQDRSSSADKRKYGDPDAFRPAPGTGLVDMSRDRNIPRSPTSPGEGFVNFDYGWFGAQTEADADKTVWTHGNHYHAPNGSLGAMHVYESKFRNWSEGYSDFDRGAYVITFIPKSWNTAPDKVKQGWPLEHHHHHH

Gabe Javitt et al. (2017) — Constitutive expression of active microbial transglutaminase in Escherichia coli and comparative characterization to a known variant

BMC Biotechnology · doi:10.1186/s12896-017-0339-4 ↗ · Activity - Classical Activity + Stability - Incubation

104 measurements ▼

Database ID

UniParc: UPI0009C2BAFA ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number (first residue absent from the mature protein)

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (104 measurements)

104 measurements — page 4 of 6

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Isopropanol	20 % v/v	100	88	69	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Isopropanol	30 % v/v	100	67	72	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Isopropanol	40 % v/v	100	38	64	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Isopropanol	50 % v/v	100	39	40	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Isopropanol	60 % v/v	100	54	57	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Isopropanol	70 % v/v	100	65	72	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Methanol	10 % v/v	100	99	94	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Methanol	20 % v/v	100	94	94	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Methanol	30 % v/v	100	116	99	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Methanol	40 % v/v	100	79	82	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Methanol	50 % v/v	100	54	72	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Methanol	60 % v/v	100	20	21	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Methanol	70 % v/v	100	23	13	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	11 % v/v	100	58	64	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	21 % v/v	100	77	62	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	31 % v/v	100	69	57	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	41 % v/v	100	56	40	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	51 % v/v	100	24	35	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	61 % v/v	100	21	42	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent
S2P	Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	70 % v/v	100	4	0	%	Digital	Continuous	356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA	6	37°C	53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine	Glycine , γ-glutamyl hydroxamate	—	—	Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Ethanol

30 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Ethanol

40 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Ethanol

50 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Ethanol

60 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Ethanol

70 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Isopropanol

10 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Isopropanol

20 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Isopropanol

30 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Isopropanol

40 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Isopropanol

50 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Isopropanol

60 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Isopropanol

70 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Methanol

10 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Methanol

20 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Methanol

30 % v/v

100

—

116

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Methanol

40 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Methanol

50 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Methanol

60 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Methanol

70 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

70 % v/v

100

—

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

—

108

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Ethanol

20 % v/v

100

—

111

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Ethanol

30 % v/v

100

—

116

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Ethanol

40 % v/v

100

—

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Isopropanol

10 % v/v

100

—

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Isopropanol

20 % v/v

100

—

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Isopropanol

30 % v/v

100

—

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Isopropanol

40 % v/v

100

—

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Isopropanol

50 % v/v

100

—

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Isopropanol

60 % v/v

100

—

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Methanol

10 % v/v

100

—

111

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Methanol

20 % v/v

100

—

128

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Methanol

30 % v/v

100

—

135

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Methanol

40 % v/v

100

—

116

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Methanol

50 % v/v

100

—

141

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Methanol

60 % v/v

100

—

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Methanol

70 % v/v

100

—

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

—

122

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Dimethyl Sulfoxide (DMSO)

20 % v/v

100

—

146

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Dimethyl Sulfoxide (DMSO)

30 % v/v

100

—

155

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Dimethyl Sulfoxide (DMSO)

40 % v/v

100

—

159

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Dimethyl Sulfoxide (DMSO)

50 % v/v

100

—

143

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Dimethyl Sulfoxide (DMSO)

60 % v/v

100

—

108

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

Activity + Stability - Incubation

Dimethyl Sulfoxide (DMSO)

70 % v/v

100

—

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Ethanol

30 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Ethanol

40 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Ethanol

50 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Ethanol

60 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Ethanol

70 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Isopropanol

10 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Isopropanol

20 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Isopropanol

30 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Isopropanol

40 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Isopropanol

50 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Isopropanol

60 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Isopropanol

70 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Methanol

10 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Methanol

20 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Methanol

30 % v/v

100

116

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Methanol

40 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Methanol

50 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Methanol

60 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Methanol

70 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

11 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

21 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

31 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

41 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

51 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

61 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, FeCl3 reagant and hydroxamate reaction product absorbance measurement, 525 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

70 % v/v

100

Digital

Continuous

356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Classical aqueous control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Ethanol

10 % v/v

100

108

105

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Ethanol

20 % v/v

100

111

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Ethanol

30 % v/v

100

116

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Ethanol

40 % v/v

100

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Isopropanol

10 % v/v

100

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Isopropanol

20 % v/v

100

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Isopropanol

30 % v/v

100

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Isopropanol

40 % v/v

100

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Isopropanol

50 % v/v

100

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Isopropanol

60 % v/v

100

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Methanol

10 % v/v

100

111

120

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Methanol

20 % v/v

100

128

122

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Methanol

30 % v/v

100

135

121

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Methanol

40 % v/v

100

116

103

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Methanol

50 % v/v

100

141

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Methanol

60 % v/v

100

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Methanol

70 % v/v

100

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

122

117

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Dimethyl Sulfoxide (DMSO)

20 % v/v

100

146

137

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Dimethyl Sulfoxide (DMSO)

30 % v/v

100

155

143

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Dimethyl Sulfoxide (DMSO)

40 % v/v

100

159

146

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Dimethyl Sulfoxide (DMSO)

50 % v/v

100

143

124

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Dimethyl Sulfoxide (DMSO)

60 % v/v

100

108

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

S2P

Activity + Stability - Incubation

Dimethyl Sulfoxide (DMSO)

70 % v/v

100

Digital

Continuous

Incubation: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA, Assay: 356 mM Tris-acetate buffer, 10 mM sodium phosphate buffer, 1.8 mM EDTA

Incubation: 6, Assay: 6

Incubation: 4°C, Assay: 37°C

53 mM benzyloxycarbonyl-glutaminyl-glycine, 352 mM Hydroxylamine

Glycine, γ-glutamyl hydroxamate

—

Water-incubated control (in %), assay in the presence of organic solvent

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Mutations in this dataset (2)

WT S2P

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Visualization : Activity + Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Structure

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Transglutaminase from Streptomyces mobaraensis

Enzyme Description

Sequence

Experimental Data (104 measurements)

Visualization : Activity — Classical

Mutation Effect

Visualization : Activity + Stability — Incubation

Mutation Effect

Structure

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