N-terminal truncated lipase from Pseudomonas sp. AMS3

Enzyme Description

Species
Pseudomonas sp. AMS3 β†—
Extremophile
No but"cold-adapted" enzyme
EC Number
3.1.1.3 β†—

Sequence

Length: 388 amino acids
ASLRANDAPIVLLHGFTGWGREEMFGFKYWGGVRGDIEQWLNDNGYRTYTLAVGPLSSNWDRACEAYAQLVGGTVDYGAAHAAKHGHARFGRTYPGLLPELKRGGRIHIIAHSQGGQTARMLVSLLENGSQEEREYAKAHNVSLSPLFEGGHHFVLSVTTIATPHDGTTLVNMVDFTDRFFDLQKAVLEAAAVASNVPYTSQVYDFKLDQWGLRRQPGESFDHYFERLKRSPVWTSTDTARYDLSVSGAEKLNQWVQASPNTYYLSFSTERTYRGALTGNHYPELGMNAFSAVVCAPFLGSYRNPTLGIDDRWLENDGIVNTVSMNGPKRGSSDRIVPYDGTLKKGVWNDMGTYNVDHLEIIGVDPNPSFDIRAFYLRLAEQLASLRP
Wahhida Latip et al. (2018) β€” The Effect of N-Terminal Domain Removal towards the Biochemical and Structural Features of a Thermotolerant Lipase from an Antarctic Pseudomonas sp. Strain AMS3
International Journal of Molecular Sciences  Β· doi:10.3390/ijms19020560 β†—  Β· Stability - Incubation
10 measurements ▼
Database ID
UniProt: A0A0K2CUU1 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (10 measurements)

10 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 25 % v/v 100 148 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: ? mM phosphate buffer Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 5Β°C 0.35 Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about pH
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase Methanol 25 % v/v 100 10 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: ? mM phosphate buffer Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 5Β°C 0.35 Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about pH
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase Acetonitrile 25 % v/v 100 9 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: ? mM phosphate buffer Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 5Β°C 0.35 Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about pH
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase Ethanol 25 % v/v 100 35 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: ? mM phosphate buffer Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 5Β°C 0.35 Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about pH
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase 1-Propanol 25 % v/v 100 52 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: ? mM phosphate buffer Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 5Β°C 0.35 Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about pH
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase Chloroform 25 % v/v 100 116 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: ? mM phosphate buffer Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 5Β°C 0.35 Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about pH
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase Benzene 25 % v/v 100 55 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: ? mM phosphate buffer Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 5Β°C 0.35 Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about pH
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase Toluene 25 % v/v 100 134 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: ? mM phosphate buffer Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 5Β°C 0.35 Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about pH
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase Xylene 25 % v/v 100 59 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: ? mM phosphate buffer Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 5Β°C 0.35 Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about pH
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase n-Hexane 25 % v/v 100 77 % Digital Continuous Incubation: 50 mM Tris-HCl buffer, Assay: ? mM phosphate buffer Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 5Β°C 0.35 Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about pH

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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