Lipase (gene LipA9) from Marinobacter lipolyticus 27-A9

Enzyme Description

Species
Marinobacter lipolyticus 27-A9 β†—
Extremophile
Yes likely psychrophilic or psychrotrophic organism (from Antarctic ocean)
EC Number
3.1.1.3 β†—

Sequence

Length: 404 amino acids
MTESKQVTGLSSGHLTHIEDHLDRRYIQPGKLPGALTLVARRGEIAYLKAQGLMDVERNKPVCRDTVFRIYSMTKPITSIAMMQLYEQGRFLLDDPVHKYIPAWKNLRVYNSGVYPNFLTTPATSTMTIRDLFTHMSGLTYGFMNRTNVDAAYRELKLDGSRNLTLEALVGHLAELPLEFSPGTAWNYSVSTDVLGYLVQLLADQPFDEYLREHIFEPLAMSDTGFHVRDDQLDRFAACYQYDPVDQFKLQDDPQTSPFRDKRRFLSGGGGLVSTIDDYFHFAQALCQGGEFGGRRIIGRKTLEFMRRNHLPGNQDLPGLSVGPFSETPYAGTGFGLGFSVKTDVAKSQINGSVGEYGWGGLASTNFIIDPVEELVVIFMTQLIPSSTYPIRQELRAIVNGALV
Se Hyeon Park et al. (2018) β€” Characterization of Organic Solvent-Tolerant Lipolytic Enzyme from Marinobacter lipolyticus Isolated from the Antarctic Ocean
Applied Biochemistry and Biotechnology  Β· doi:10.1007/s12010-018-2865-5 β†—  Β· Stability - Incubation
54 measurements ▼
Database ID
UniParc: UPI000EF332DA β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (54 measurements)

54 measurements β€” page 2 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 40 % v/v 100.0 102.0 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 50 % v/v 100.0 92.0 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 60 % v/v 100.0 94.0 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 70 % v/v 100.0 95.0 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 80 % v/v 100.0 73.0 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 90 % v/v 100.0 46.0 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 95 % v/v 100.0 41.0 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 40 % v/v 100.0 97.0 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 30 % v/v 100.0 96.8 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetonitrile 30 % v/v 100.0 70.5 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 30 % v/v 100.0 93.2 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 30 % v/v 100.0 100.0 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1,2-Dimethoxyethane 30 % v/v 100.0 94.7 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 30 % v/v 100.0 100.5 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Propionitrile 30 % v/v 100.0 104.2 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 2-Butanone 30 % v/v 100.0 93.2 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Butanol 30 % v/v 100.0 113.2 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Diethyl Ether 30 % v/v 100.0 115.3 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10 % v/v 100.0 103.0 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 20 % v/v 100.0 100.0 % Digital Continuous Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 37Β°C 0.1 mM p-Nitrophenyl hexanoate caproic acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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