Chitinase (gene mtch509) from Microbulbifer thermotolerans DAU221

Enzyme Description

Species
Microbulbifer thermotolerans DAU221 β†—
Extremophile
No but "thermotolerant" organism
EC Number
3.2.1.14 β†—

Sequence

Length: 509 amino acids
MKMFWLGLTALGLCAANAAAVDCRSLPDWENAGVYVGGDQVRHLNNAYTAKWWTQAEDPATHSGTWDVWRYDGACDGVSSSSSSSSSSSSSSSSGSGSSSGSSSSGGSCSSPQYVDGGRYSTGDLVQNVGSEFRCDVGGWCSLGGAYAPGVGWAWGEAWTQLGDCEGSSSSSSSSSGSSGSSGNPGSSGGNSGLLPKHALVGYWHNFNNGSGLYRIAEVSDVWDVIVVAFADDAGNGNVAFNLDAGLNKQQFIDDIAAKRAAGKIVVLSFGGQNGTVTLNSAEHVTNFVNSTADILDEYGFDGIDIDLESGAGVMHGAPVVQNLVSAVKQLKARYPNMYLSMAPEHPYVQGGYVAYTGIWGAYLPMIDQLRDELDLLHVQLYNNGGLSTPYHSQAYAAGSVDMMVASAMMLIEGFPLGGGSAGYFDGLRPDQVALGLPSGPSAAGSGQAATADINRALDCLTARVNCGSLTPSQAWPDFNGVMTWSINWDMHDGGIFSGPVGSHVHNLP
Hyo-Jung Lee et al. (2018) β€” Cloning, purification, and characterization of an organic solvent-tolerant chitinase, MtCh509, from Microbulbifer thermotolerans DAU221
Biotechnology for Biofuels  Β· doi:10.1186/s13068-018-1299-1 β†—  Β· Activity - Classical
22 measurements ▼
Database ID
UniProt: A0A143HNF3 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (22 measurements)

22 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20 % v/v 100 158 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent n-Hexane 20 % v/v 100 173 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent Toluene 20 % v/v 100 157 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent Benzene 20 % v/v 100 201 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent Isoamyl Alcohol 20 % v/v 100 103 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent 1-Butanol 20 % v/v 100 77 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent Isopropanol 20 % v/v 100 16 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent Acetonitrile 20 % v/v 100 6 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent Ethanol 20 % v/v 100 19 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent Acetone 20 % v/v 100 9 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent Methanol 20 % v/v 100 9 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100 139 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent n-Hexane 10 % v/v 100 139 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent Toluene 10 % v/v 100 165 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent Benzene 10 % v/v 100 148 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent Isoamyl Alcohol 10 % v/v 100 127 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent 1-Butanol 10 % v/v 100 74 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent Isopropanol 10 % v/v 100 33 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent Acetonitrile 10 % v/v 100 20 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent Ethanol 10 % v/v 100 44 % Direct Continuous 50 mM citrate buffer 5 55Β°C 0.01 Colloidal Chitin Reducing sugars β€” β€” Classical aqueous control (in %)
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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