Nitrilase (gene PaCNit) from Pannonibacter carbonis Q4.6

Enzyme Description

Species
Pannonibacter carbonis Q4.6 β†—
Extremophile
No but thermostable enzyme
EC Number
3.5.5.1 β†—

Sequence

Length: 297 amino acids
MAEFPIALCSLNLGFAPALPEAYVAAIDQRMARAKADGARLLMLPEYCIEACLAFRPEALEPTAEMAFLSEVAVAIEPQLAALAARHGLSLLAGSMPWPHEGGGHTNRAIFFTPDGCRHVHDKLSLTPFEKAPEPWVLTPGRKLTVFEVEGVRIAMLICLDVEMPALSCLLAKEHLDLLLVPSMTGKLSGYHRVYDCAKARAVELLTSVALCGCTGVATGTTQNDTNFSGAALYVPCEAELGYTGTAVSLTPTGGEKGEEPYVVVHPPIDAVRRMRAGAAEVWPGAWSAAGIEICRQ
Dejian Liu et al. (2019) β€” Cloning, expression, and characterization of a novel nitrilase, PaCNit, from Pannonibacter carbonis Q4.6
Biotechnology Letters  Β· doi:10.1007/s10529-019-02661-x β†—  Β· Activity - Classical
22 measurements ▼
Database ID
UniParc: UPI000D111D56 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (22 measurements)

22 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) Glycerol 5 % v/v 100 48 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) Dimethyl Sulfoxide (DMSO) 5 % v/v 100 48 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) Methanol 5 % v/v 100 71 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) Acetone 5 % v/v 100 120 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) Diethyl Ether 5 % v/v 100 77 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) n-Heptane 5 % v/v 100 75 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) Ethyl Acetate 5 % v/v 100 65 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) Chloroform 5 % v/v 100 98 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) Ethanol 5 % v/v 100 93 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) n-Hexane 5 % v/v 100 94 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) Isopropanol 5 % v/v 100 99 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) Glycerol 1 % v/v 100 132 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) Dimethyl Sulfoxide (DMSO) 1 % v/v 100 68 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) Methanol 1 % v/v 100 87 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) Acetone 1 % v/v 100 89 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) Diethyl Ether 1 % v/v 100 91 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) n-Heptane 1 % v/v 100 92 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) Ethyl Acetate 1 % v/v 100 94 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) Chloroform 1 % v/v 100 97 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) Ethanol 1 % v/v 100 97 % Direct Continuous 50 mM sodium phosphate buffer 7.2 30Β°C 50 mM 3-Cyanopyridine Ammonia , Nicotinic acid β€” β€” Correct contol (in %)
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Visualization : Activity β€” Classical

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Structure

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