Lipase from Pseudomonas cepacia

Enzyme Description

Species

Extremophile

EC Number

3.1.1.3 ↗

Sequence

Length: 364 amino acids

MARTMRSRVVAGAVACAMSIAPFAGTTAVMTLATTHAAMAATAPAAGYAATRYPIILVHGLSGTDKYAGVLEYWYGIQEDLQQNGATVYVANLSGFQSDDGPNGRGEQLLAYVKTVLAATGATKVNLVGHSQGGLSSRYVAAVAPDLVASVTTIGTPHRGSEFADFVQDVLAYDPTGLSSSVIAAFVNVFGILTSSSHNTNQDALAALQTLTTARAATYNQNYPSAGLGAPGSCQTGAPTETVGGNTHLLYSWAGTAIQPTLSVFGVTGATDTSTLPLVDPANVLDLSTLALFGTGTVMINRGSGQNDGLVSKCSALYGKVLSTSYKWNHLDEINQLLGVRGAYAEDPVAVIRTHANRLKLAGV

Satish C. Mohapatra et al. (1997) — Lipase kinetics in organic-water solvent with amphipathic substrate for chiral reaction

Biotechnology and Bioengineering · doi:10.1002/(SICI)1097-0290(19970720)55:2%3C399::AID-BIT17%3E3.0.CO;2-C ↗ · Activity - Classical

26 measurements ▼

Database ID

UniProt: P22088 ↗

Sequence Annotation

Inferred

Protein Source

Commercially purchased

Experimental Data (26 measurements)

26 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Initial reaction rate (in µM/min) measured by chromatography	Acetone	25 % v/v	11.2	34.0	µM/min	Digital	Continuous	0.01 M phosphate buffer	7.1	?	2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane	1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane , Acetate	—	—	Classical aqueous control (in microM/min)
Activity - Classical	Initial reaction rate (in µM/min) measured by chromatography	Acetone	15 % v/v	11.2	31.5	µM/min	Digital	Continuous	0.01 M phosphate buffer	7.1	?	2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane	1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane , Acetate	—	—	Classical aqueous control (in microM/min)
Activity - Classical	Initial reaction rate (in µM/min) measured by chromatography	Tetrahydrofuran (THF)	25 % v/v	11.2	19.0	µM/min	Direct	Continuous	0.01 M phosphate buffer	7.1	?	2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane	1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane , Acetate	—	—	Classical aqueous control (in microM/min)
Activity - Classical	Initial reaction rate (in µM/min) measured by chromatography	Acetone	25 % v/v	11.2	31.7	µM/min	Direct	Continuous	0.01 M phosphate buffer	7.1	?	2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane	1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane , Acetate	—	—	Classical aqueous control (in microM/min)
Activity - Classical	Initial reaction rate (in µM/min) measured by chromatography	Acetonitrile	25 % v/v	11.2	22.16	µM/min	Direct	Continuous	0.01 M phosphate buffer	7.1	?	2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane	1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane , Acetate	—	—	Classical aqueous control (in microM/min)
Activity - Classical	Initial reaction rate (in µM/min) measured by chromatography	Dimethylformamide (DMF)	25 % v/v	11.2	79.17	µM/min	Direct	Continuous	0.01 M phosphate buffer	7.1	?	2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane	1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane , Acetate	—	—	Classical aqueous control (in microM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

100 % v/v

11.2

0.0

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

100 % v/v

35.3

0.4

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

18 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

75 % v/v

35.3

9.1

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

18 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

50 % v/v

35.3

37.0

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

18 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

35 % v/v

35.3

56.8

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

18 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

25 % v/v

35.3

71.3

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

18 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

15 % v/v

35.3

63.5

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

18 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

100 % v/v

15.8

0.0

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

9 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

75 % v/v

15.8

1.2

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

9 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

50 % v/v

15.8

17.4

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

9 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

35 % v/v

15.8

49.0

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

9 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

25 % v/v

15.8

55.6

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

9 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

15 % v/v

15.8

49.0

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

9 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by HPLC in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25 % v/v

11.2

82.3

µM/min

Direct

Continuous

0.01 M phosphate buffer

7.1

2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

75 % v/v

11.2

0.0

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

50 % v/v

11.2

0.0

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

45 % v/v

11.2

0.0

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

40 % v/v

11.2

24.5

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

35 % v/v

11.2

30.7

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

30 % v/v

11.2

31.1

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

25 % v/v

11.2

34.0

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

15 % v/v

11.2

31.5

µM/min

Digital

Continuous

0.01 M phosphate buffer

7.1

2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Tetrahydrofuran (THF)

25 % v/v

11.2

19.0

µM/min

Direct

Continuous

0.01 M phosphate buffer

7.1

2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetone

25 % v/v

11.2

31.7

µM/min

Direct

Continuous

0.01 M phosphate buffer

7.1

2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Acetonitrile

25 % v/v

11.2

22.16

µM/min

Direct

Continuous

0.01 M phosphate buffer

7.1

2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

Activity - Classical

Initial reaction rate (in µM/min) measured by chromatography

Dimethylformamide (DMF)

25 % v/v

11.2

79.17

µM/min

Direct

Continuous

0.01 M phosphate buffer

7.1

2.5 mM 1-chloro-2-acetoxy-3-(1-naphthyloxy)-propane

1-chloro-2-hydroxy-3-(1-naphthyloxy)-propane, Acetate

—

Classical aqueous control (in µM/min)

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Lipase from Pseudomonas cepacia

Enzyme Description

Sequence

Experimental Data (26 measurements)

Visualization : Activity — Classical

Structure

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