Ξ±-amylase (gene TfAmy48) from Tepidimonas fonticaldi HB23

Enzyme Description

Species
Tepidimonas fonticaldi HB23 β†—
Extremophile
Yes "thermophilic" organism
EC Number
3.2.1.1 β†—

Sequence

Length: 443 amino acids
DAIVHAFNWKYSRVTANAEHKAAAGYKNVLVSPAMKSSGYEWWARHQPQDLRVIDSPIGNKTDFKAMVNALRNHGVGVYTDVVLNHMANETWKRTGLNYPGSEILTNYANQASYYQQQTLFGDLTQNLFSVHDFHPWGCITDWSNPGHVQYWRLCGGPGDRGLPDLAANDWVLEQQKAYLRALKQIGITGFRIDAVKHMYQYHIDQVFTPDIIADMHVFREVITSGGLGDATYDAFLAPYLNNTNHAAYDFDLFASIRAAFSYQGSMTLLHDPQAYGQALDNARAVTFTITHDIPTNDGFRYQILDPQDEQLAYAYILGKDGGTPLIYSDNLAANEDRDYGRWANVWNQPTMLKMLRFHNAMQGQSMTMIHSDPCLLVGKRGKQGEPGINKCGEAKPITIDTYQHEFNWYRAYQDVLQDDEFIVNNRYHTLSIPARSARMWMQ
Fawzi Allala et al. (2019) β€” Purification, biochemical, and molecular characterization of a novel extracellular thermostable and alkaline Ξ±-amylase from Tepidimonas fonticaldi strain HB23
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2019.03.201 β†—  Β· Stability - Incubation
20 measurements ▼
Database ID
UniProt: A0A482EWM9 β†—
Sequence Annotation
Explicit (first 23 amino acids from UniProt sequence removed)
Protein Source
Purified, bacterium Thermofilum fonticaldi strain HB23

Experimental Data (20 measurements)

20 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Hexanol 25 % v/v 100 102 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 25 % v/v 100 110 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Dimethylformamide (DMF) 25 % v/v 100 154 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Methanol 25 % v/v 100 104 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethanol 25 % v/v 100 115 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetonitrile 25 % v/v 100 32 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isopropanol 25 % v/v 100 95 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isopropanol 25 % v/v 100 106 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethyl Acetate 25 % v/v 100 45 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Butanol 25 % v/v 100 101 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase n-Hexadecane 25 % v/v 100 99 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Chloroform 25 % v/v 100 140 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Benzene 25 % v/v 100 45 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Toluene 25 % v/v 100 101 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Cyclohexane 25 % v/v 100 112 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase n-Hexane 25 % v/v 100 104 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase n-Heptane 25 % v/v 100 73 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase n-Octane 25 % v/v 100 91 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isooctane 25 % v/v 100 88 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase n-Decane 25 % v/v 100 80 % Digital Continuous Incubation: ?, Assay: 40 mM HEPES buffer, 4 mM CaCl2 Incubation: ?, Assay: 8 Incubation: 40Β°C, Assay: 80Β°C 8 mg/ml Starch Reducing sugars β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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