Esterase (gene EstGtA3) from Geobacillus thermodenitrificans NG80‐2

Enzyme Description

Species
Geobacillus thermodenitrificans NG80‐2 β†—
Extremophile
Yes "thermophilic" organism
EC Number
3.1.1.1 β†—

Sequence

Length: 244 amino acids
MVMAIAAGIMRDYALYSGELQEKIELLVYLPSNFSPLHKYSLLIAQDGKDYFMYGKVKRVIETLMEEGTIDRTIVVGIPYRNVNDRYEKYHPAGQKHEAYIRFLAHELVPFLDHELPTYQMGKGRALIGDSLGGTISLLAGLLYPHTFGNIAMQSPYIDDSVLARIRAFRDPSLLSIYHSVGTEETAVKTTDGHVRDFITPNRVARDLFMEKQFAYTYHEFAGGHAWTYWQPDLPRAVSAILSL
Nicola Curci et al. (2019) β€” Identification of a novel esterase from the thermophilic bacterium Geobacillus thermodenitrificans NG80-2
Extremophiles  Β· doi:10.1007/s00792-019-01093-9 β†—  Β· Stability - Incubation
20 measurements ▼
Database ID
UniProt: A4ILC0 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (20 measurements)

20 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Chloroform 10 % v/v 100 51 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethylformamide (DMF) 30 % v/v 100 60 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethylformamide (DMF) 10 % v/v 100 49 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetonitrile 30 % v/v 100 42 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetonitrile 10 % v/v 100 41 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Butanol 30 % v/v 100 42 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Butanol 10 % v/v 100 37 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 30 % v/v 100 53 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 10 % v/v 100 30 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Chloroform 30 % v/v 100 34 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 10 % v/v 100 92 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 30 % v/v 100 62 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10 % v/v 100 93 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 30 % v/v 100 62 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 10 % v/v 100 89 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase n-Hexane 30 % v/v 100 143 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase n-Hexane 10 % v/v 100 113 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 30 % v/v 100 40 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 10 % v/v 100 85 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 30 % v/v 100 50 % Digital Continuous Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol Incubation: ?, Assay: 7.5 Incubation: 55Β°C, Assay: 55Β°C 2 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate Β· Scroll to zoom Β· Right-click drag to pan Β· Powered by Mol*