GH10 endo-1,4-Ξ²-xylanase (gene TnXynB) from Thermotoga naphthophila RKU-10T

Enzyme Description

Species
Thermotoga naphthophila RKU-10T β†—
Extremophile
Yes "hyperthermophilic" organism
EC Number
3.2.1.8 β†—

Sequence

Length: 344 amino acids
MKILPSVLILLLGCVPVFSSQNVSLRELAEKLNIYIGFAAVNNFWSLPDAEKYMEIARREFNILTHENQMKWDTIHPERDRYNFTPAEKHVEFAEENGMIVHGHTLVWHNQLPGWLTGREWTREELLNVLEDHIKTVVSHFKGRVKIWDVVNEAVSDSGTYRESVWYKTIGPEYIEKAFRWAKEADPDAILIYNDYSIEEINAKSNFVYNMIKELKEKGVPVDGIGFQMHIDHRGLNYDSFRRNLERFAELGLQIYITEMDVRIPLSGSEEYYLKKQAEVCAKIFEICLKNPAVKAIQFWGFTDKYSWVPGFFKGYGKALLFDENYNPKPCYYAIKEVLEKKRK
Ikram ul Haq et al. (2019) β€” Insight into kinetics and thermodynamics of a novel hyperstable GH family 10 endo-1,4-Ξ²-xylanase (TnXynB) with broad substrates specificity cloned from Thermotoga naphthophilaRKU-10T
Enzyme and Microbial Technology  Β· doi:10.1016/j.enzmictec.2019.04.009 β†—  Β· Stability - Incubation
11 measurements ▼
Database ID
UniProt: D2C759 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (11 measurements)

11 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetone 50 % v/v 100.0 52.3 % Direct Continuous Incubation: ?, Assay: 50% McIlvaine buffer Incubation: ?, Assay: 6 Incubation: 70Β°C, Assay: 95Β°C 2% w/v Soluble birchwood xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetonitrile 50 % v/v 100.0 54.8 % Direct Continuous Incubation: ?, Assay: 50% McIlvaine buffer Incubation: ?, Assay: 6 Incubation: 70Β°C, Assay: 95Β°C 2% w/v Soluble birchwood xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethyl Acetate 50 % v/v 100.0 56.2 % Direct Continuous Incubation: ?, Assay: 50% McIlvaine buffer Incubation: ?, Assay: 6 Incubation: 70Β°C, Assay: 95Β°C 2% w/v Soluble birchwood xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Glycerol 50 % v/v 100.0 45.41 % Direct Continuous Incubation: ?, Assay: 50% McIlvaine buffer Incubation: ?, Assay: 6 Incubation: 70Β°C, Assay: 95Β°C 2% w/v Soluble birchwood xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50 % v/v 100.0 50.3 % Direct Continuous Incubation: ?, Assay: 50% McIlvaine buffer Incubation: ?, Assay: 6 Incubation: 70Β°C, Assay: 95Β°C 2% w/v Soluble birchwood xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Dimethylformamide (DMF) 50 % v/v 100.0 44.7 % Direct Continuous Incubation: ?, Assay: 50% McIlvaine buffer Incubation: ?, Assay: 6 Incubation: 70Β°C, Assay: 95Β°C 2% w/v Soluble birchwood xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Chloroform 50 % v/v 100.0 59.12 % Direct Continuous Incubation: ?, Assay: 50% McIlvaine buffer Incubation: ?, Assay: 6 Incubation: 70Β°C, Assay: 95Β°C 2% w/v Soluble birchwood xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Methanol 50 % v/v 100.0 49.3 % Direct Continuous Incubation: ?, Assay: 50% McIlvaine buffer Incubation: ?, Assay: 6 Incubation: 70Β°C, Assay: 95Β°C 2% w/v Soluble birchwood xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethanol 50 % v/v 100.0 46.8 % Direct Continuous Incubation: ?, Assay: 50% McIlvaine buffer Incubation: ?, Assay: 6 Incubation: 70Β°C, Assay: 95Β°C 2% w/v Soluble birchwood xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Butanol 50 % v/v 100.0 44.8 % Direct Continuous Incubation: ?, Assay: 50% McIlvaine buffer Incubation: ?, Assay: 6 Incubation: 70Β°C, Assay: 95Β°C 2% w/v Soluble birchwood xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isopropanol 50 % v/v 100.0 42.8 % Direct Continuous Incubation: ?, Assay: 50% McIlvaine buffer Incubation: ?, Assay: 6 Incubation: 70Β°C, Assay: 95Β°C 2% w/v Soluble birchwood xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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