GH3 Ξ²-glucosidase (gene MtBgl85) from Microbulbifer thermotolerans DAU221

Enzyme Description

Species
Microbulbifer thermotolerans DAU221 β†—
Extremophile
No
EC Number
3.2.1.21 β†—

Sequence

Length: 772 amino acids
MKKYPIYIAAMAGLLSLAATLPLQAANESVATTQSHSQQNAIESRIQSLLAQMSVQEKIGQLALRDWGTFKAEDMKDIKQAIREGRVGGFLNVNFSSVDNNAFEELQRLAVEESPRGIPLLFGQDVIHGYETIFPIPLGQAASWNPELIRNGARIAAQEASADGIRWTFAPMIDISRDPRWGRIAETLGEDPLLTSILGVAMVEGFQTDNLSDPTSLAACGKHFAGYGAAEGGRDYNSAYIPERLLRDIYLPPFKAGIDAGMQSIMSTYSSLNDVPGTGSPFLFKQILRDEWGFDGFVVSDWNAVMEMIPHGYARDAKHAAALAANAGIDMEMHTDTYEQFLARLMDEGEFTEAQLDTAVANILRVKLRLGLWENPYPRAKTPAEREQIIRNDQFLNAAKEAAKETFVLLKNDKQLLPLKKGQTVALIGPLADAPHEQMGTWVYNGNKEHSRTLLPALRAMVGDKGKILYAAGLEYSRDTSTRGFKAAIKAAKKADVVLFVGGEEAILSGEGHSRGDIRLPGAQAQLLAELAKTGKPLAMVLMAGRPLQLDQTLEQADAVMMAWHPGTMAGPALAEVLYGETSPSGRLPLSWPVGAGQIPIYYNHLPTGRPATDDNYTRIEKIGREVFQHQPGNSSNLLDYGHKPLFPFGYGLTYSEFHYSELELSDSTLDADGQLHVSATITNTGKRTATETVQLYVRDLVGSVSRPVRELKGFERITLAPGESRRVSFTLKANQLAFHNAQMQQVIEPGEFHVWIAPDAESGLQGSFTLQ
Hyo-Min Pyeon et al. (2019) β€” Cloning, purification, and characterization of GH3 Œ≀ -glucosidase, MtBgl85, from Microbulbifer thermotolerans DAU221
PeerJ  Β· doi:10.7717/peerj.7106 β†—  Β· Activity - Classical
33 measurements ▼
Database ID
UniParc: UPI0008E19425 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (33 measurements)

33 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 30 % v/v 100 1 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent 1-Butanol 20 % v/v 100 48 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Isoamyl Alcohol 20 % v/v 100 85 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Benzene 20 % v/v 100 72 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Toluene 20 % v/v 100 115 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent n-Hexane 20 % v/v 100 99 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30 % v/v 100 0 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 30 % v/v 100 2 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetonitrile 30 % v/v 100 0 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Isopropanol 20 % v/v 100 22 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetone 30 % v/v 100 0 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Isopropanol 30 % v/v 100 1 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent 1-Butanol 30 % v/v 100 99 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Isoamyl Alcohol 30 % v/v 100 139 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Benzene 30 % v/v 100 116 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Toluene 30 % v/v 100 103 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent n-Hexane 30 % v/v 100 88 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100 79 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetone 20 % v/v 100 12 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 20 % v/v 100 15 % Direct Continuous 20 mM sodium phosphate buffer 7 50Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Correct contol (in %)
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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