Laccase 10 (gene LACC10) from Pleurotus ostreatus HAUCC 162

Enzyme Description

Species
Pleurotus ostreatus HAUCC 162 β†—
Extremophile
No
EC Number
1.10.3.2 β†—

Sequence

Length: 533 amino acids
MFPGARILATLTLALHLLHGTHAAIGPAGNMYIVNEDVSPDGFARSAVVARSVPATDPTPATASIPGVLVQGNKGDNFQLNVVNQLSDTTMLKTTSIHWHGFFQAGSSWADGPAFVTQCPVASGDSFLYNFNVPDQAGTFWYHSHLSTQYCDGLRGPFVVYDPSDPHLSLYDIDNADTVITLEDWYHIVAPQNAAIPTPDSTLINGKGRYAGGPTSPLAIINVESNKRYRFRLVSMSCDPNFTFSIDGHSLLVIEADAVNIVPITVDSIQIFAGQRYSFVLTANQAVDNYWIRANPNLGSTGFVGGINSAILRYAGATEDDPTTTSSTSTPLLETNLVPLENPGAPGPPVPGGADININLAMAFDFTTFELTINGVPFLPPTAPVLLQILSGASTAASLLPSGSIYELEANKVVEISMPALAVGGPHPFHLHGHTFDVIRSAGSTTYNFDTPARRDVVNTGTGANDNVTIRFVTDNPGPWFLHCHIDWHLEIGLAVVFAEDVTSISAPPAAWDDLCPIYNALSDNDKGGIVPS
Rui Zhuo et al. (2019) β€” The roles of Pleurotus ostreatus HAUCC 162 laccase isoenzymes in decolorization of synthetic dyes and the transformation pathways
Chemosphere  Β· doi:10.1016/j.chemosphere.2019.06.113 β†—  Β· Activity - Classical Stability - Incubation
80 measurements ▼
Database ID
UniProt: A0A1S6YJF7 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host yeast Pichia pastoris GS115

Experimental Data (80 measurements)

80 measurements β€” page 4 of 4
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (6 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetone 50 % v/v 100.0 81.03 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (6 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10 % v/v 100.0 94.36 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (6 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50 % v/v 100.0 87.47 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (6 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetonitrile 10 % v/v 100.0 95.89 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (6 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetonitrile 50 % v/v 100.0 94.1 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetone 50 % v/v 100.0 65.35 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (6 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Dimethylformamide (DMF) 50 % v/v 100.0 95.42 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (6 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Ethylene Glycol 10 % v/v 100.0 98.56 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (6 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Ethylene Glycol 50 % v/v 100.0 91.16 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (6 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Glycerol 10 % v/v 100.0 99.59 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (6 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Glycerol 50 % v/v 100.0 99.98 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Methanol 10 % v/v 100.0 65.66 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Methanol 50 % v/v 100.0 64.06 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Ethanol 10 % v/v 100.0 62.88 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Ethanol 50 % v/v 100.0 76.89 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase 1-Propanol 10 % v/v 100.0 63.95 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase 1-Propanol 50 % v/v 100.0 26.37 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Isopropanol 10 % v/v 100.0 70.57 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Isopropanol 50 % v/v 100.0 61.89 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetone 10 % v/v 100.0 73.33 % Direct Continuous Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer Incubation: 4.8, Assay: 5 Incubation: 4Β°C, Assay: 25Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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