Pyruvate decarboxylase from Zymobacter palmae

Enzyme Description

Species
Zymobacter palmae β†—
Extremophile
No
EC Number
4.1.1.1 β†—

Sequence

Length: 556 amino acids
MYTVGMYLAERLAQIGLKHHFAVAGDYNLVLLDQLLLNKDMEQVYCCNELNCGFSAEGYARARGAAAAIVTFSVGAISAMNAIGGAYAENLPVILISGSPNTNDYGTGHILHHTIGTTDYNYQLEMVKHVTCARESIVSAEEAPAKIDHVIRTALRERKPAYLEIACNVAGAECVRPGPINSLLRELEVDQTSVTAAVDAAVEWLQDRQNVVMLVGSKLRAAAAEKQAVALADRLGCAVTIMAAEKGFFPEDHPNFRGLYWGEVSSEGAQELVENADAILCLAPVFNDYATVGWNSWPKGDNVMVMDTDRVTFAGQSFEGLSLSTFAAALAEKAPSRPATTQGTQAPVLGIEAAEPNAPLTNDEMTRQIQSLITSDTTLTAETGDSWFNASRMPIPGGARVELEMQWGHIGWSVPSAFGNAVGSPERRHIMMVGDGSFQLTAQEVAQMIRYEIPVIIFLINNRGYVIEIAIHDGPYNYIKNWNYAGLIDVFNDEDGHGLGLKASTGAELEGAIKKALDNRRGPTLIECNIAQDDCTETLIAWGKRVAATNSRKPQA
Samuel Sutiono et al. (2019) β€” To beat the heat–engineering of the most thermostable pyruvate decarboxylase to date
RSC Advances  Β· doi:10.1039/c9ra06251c β†—  Β· Stability - Incubation
8 measurements ▼
Database ID
UniProt: Q8KTX6 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (8 measurements)

8 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase Ethanol 4 % v/v 100 98 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 50Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
Stability - Incubation Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase Ethanol 8 % v/v 100 94 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 50Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
Stability - Incubation Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase Ethanol 12 % v/v 100 87 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 50Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
Stability - Incubation Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase Ethanol 16 % v/v 100 66 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 50Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
Stability - Incubation Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase Ethanol 20 % v/v 100 15 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 50Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
Stability - Incubation Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase Ethanol 24 % v/v 100 10 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 50Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
Stability - Incubation Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase Ethanol 28 % v/v 100 10 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 50Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
Stability - Incubation Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase Ethanol 32 % v/v 100 5 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 50Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume. β€” β€” β€” Reference value. Hover for details.

Structure

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