N-terminal truncated (54 residues) esterase (gene estA1) from Alteromonas sp. 39-G1

Enzyme Description

Species

Extremophile

EC Number

3.1.1.1 ↗

Sequence

Length: 325 amino acids

PAKTLPLPSASSDELKSAISQYPMPSVDEVINNTPQSIEQWRELIQIRNADQKKKIKKMRKQFDVDVSLEKINGVPVRRLTPKTIAPEFKNKVFIDVHGGAYVFFSGLPSIEESLLIAHRVGITVISIDYSMPPHAPFPAALNDVVSVYSSVAAEHGAQNLFIGGTSAGAGLVLAAVQTLIADKQPLPAAVYAGTPWADLTKTGDTLYTNEGVDRILVTYQGFLEAAANLYAGSESLTHSSISPLYGNFDGFPPTFLISGTRDMFLSDTVRVNRKLRDAQVRTQLEVFEGLSHADYVVAYETPESHSVYQELKQFLLSVCTKSSD

Seok-Jae Won et al. (2020) — Characterization of Novel Salt-Tolerant Esterase Isolated from the Marine Bacterium Alteromonas sp. 39-G1

Journal of Microbiology and Biotechnology · doi:10.4014/jmb.1907.07057 ↗ · Stability - Incubation

24 measurements ▼

Database ID

UniParc: UPI000C1070DA ↗

Sequence Annotation

Explicit (first 54 residues from UniProt sequence removed)

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (24 measurements)

24 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	15 % v/v	100	112	%	Direct	Continuous	Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 40°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	15 % v/v	100	86	%	Direct	Continuous	Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 40°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	15 % v/v	100	39	%	Direct	Continuous	Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 40°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	15 % v/v	100	83	%	Direct	Continuous	Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 40°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Acetone

30 % v/v

100

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

n-Hexane

50 % v/v

100

131

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Cyclohexane

50 % v/v

100

147

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Isopropanol

50 % v/v

100

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Acetone

50 % v/v

100

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Ethanol

50 % v/v

100

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Acetonitrile

50 % v/v

100

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Methanol

50 % v/v

100

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

50 % v/v

100

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

n-Hexane

30 % v/v

100

119

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Cyclohexane

30 % v/v

100

127

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Isopropanol

30 % v/v

100

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

15 % v/v

100

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Ethanol

30 % v/v

100

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Acetonitrile

30 % v/v

100

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Methanol

30 % v/v

100

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

30 % v/v

100

101

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

n-Hexane

15 % v/v

100

108

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Cyclohexane

15 % v/v

100

119

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Isopropanol

15 % v/v

100

103

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Acetone

15 % v/v

100

112

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Ethanol

15 % v/v

100

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Acetonitrile

15 % v/v

100

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Methanol

15 % v/v

100

Direct

Continuous

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 40°C

0.1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

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Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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N-terminal truncated (54 residues) esterase (gene estA1) from Alteromonas sp. 39-G1

Enzyme Description

Sequence

Experimental Data (24 measurements)

Visualization : Stability — Incubation

Structure

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