Ene-reductase (gene FOYE-1) from Ferrovum sp. JA12

Enzyme Description

Species
Ferrovum sp. JA12 β†—
Extremophile
Yes "acidophilic" organism
EC Number
1.3.1.31 β†—

Sequence

Length: 375 amino acids
MGHHHHHHHHHHSSGHIEGRHMSLLFSPYQLGSLSLANRLVIAPMCQYSAVDGIAQDWHLMHLGRLAISGAGLVIVEATGVNPEGRITPFCLGLYNDEQEAALGRIVAFAREFGQAKMAIQLAHAGRKASTRRPWDPGSPYSPEEGGWQTWAPSAIKFYEESLTPHPMSIEDLETVKQDFVNSAIRAERAGFKAIELHGAHGYLIHQFLSPLSNQRQDQYGGSLENRMRYPLEILSAVKHALSAEMVVGMRISAVDWAPGGLTIEESITFSQECEKRGAGFIHVSTGGLVAHQQIPVGPGYQVEHAQAIKQNVNIPTMAVGLITHSAQAETILKSEQADMIAIARAALKNPHWPWTAALELGDKPFAPPQYQRAR
Dirk Tischler et al. (2020) β€” Asymmetric Reduction of ( R )-Carvone through a Thermostable and Organic-Solvent-Tolerant Ene-Reductase
ChemBioChem  Β· doi:10.1002/cbic.201900599 β†—  Β· Activity - Classical
63 measurements ▼
Database ID
UniParc: UPI00070287B1 β†—
Sequence Annotation
Explicit - Provided (hist-tag included)
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (63 measurements)

63 measurements β€” page 3 of 4
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 30 % v/v 100 88 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 40 % v/v 100 62 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 50 % v/v 100 30 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 60 % v/v 100 0 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Acetone 10 % v/v 100 120 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Acetone 20 % v/v 100 114 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Acetone 30 % v/v 100 96 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Acetone 40 % v/v 100 50 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 10 % v/v 100 104 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Acetone 60 % v/v 100 0 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Isopropanol 10 % v/v 100 110 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Isopropanol 20 % v/v 100 108 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Isopropanol 30 % v/v 100 86 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Isopropanol 40 % v/v 100 67 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Isopropanol 50 % v/v 100 31 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Isopropanol 60 % v/v 100 16 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100 103 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20 % v/v 100 86 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30 % v/v 100 48 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 40 % v/v 100 14 % Digital Continuous 50 mM phosphate buffer 7.1 22Β°C 1 mM N-Methylmaleimide N-Methylsuccinimide FMN, electron donor: 0.2 mM NADPH β€” Classical aqueous control (in %)
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Visualization : Activity β€” Classical

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Structure

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