Lipase (gene BTL2) from Bacillus thermocatenulatus

Enzyme Description

Species
Bacillus thermocatenulatus β†—
Extremophile
Yes thermophilic organism, "thermoalkalophilic" enzyme
EC Number
3.1.1.3 β†—

Sequence

Length: 388 amino acids
ASPRANDAPIVLLHGFTGWGREEMLGFKYWGGVRGDIEQWLNDNGYRTYTLAVGPLSSNWDRACEAYAQLVGGTVDYGAAHAAKHGHARFGRTYPGLLPELKRGGRVHIIAHSQGGQTARMLVSLLENGSQEEREYAKAHNVSLSPLFEGGHHFVLSVTTIATPHDGTTLVNMVDFTDRFFDLQKAVLKAAAVASNVPYTSQVYDFKLDQWGLRRQPGESFDHYFERLKRSPVWTSTDTARYDLSIPGAEKLNQWVQASPNTYYLSFSTERTHRGALTGNYYPELGMNAFSAVVCAPFLGSYRNEALGIDDRWLENDGIVNTVSMNGPKRGSSDRIVPYDGTLKKGVWNDMGTCNVDHLEVIGVDPNPSFDIRAFYLRLAEQLASLRP
Mohamed Shehata et al. (2020) β€” Understanding thermal and organic solvent stability of thermoalkalophilic lipases: insights from computational predictions and experiments
Journal of Molecular Modeling  Β· doi:10.1007/s00894-020-04396-3 β†—  Β· Stability - Incubation
56 measurements ▼
Database ID
UniProt: Q59260 β†—
Sequence Annotation
Explicit - Provided PDB Accession Number (first 29 residues from UniProt sequence absent from the mature protein)
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (56 measurements)

56 measurements β€” page 3 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase Methanol 40 % v/v 100 165 % Digital Continuous Incubation: ?, Assay: 25 mM sodium phosphate buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C ? mM 4-Methylumbelliferyl butyrate 4-Methylumbelliferone , Butyric Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase Methanol 50 % v/v 100 138 % Digital Continuous Incubation: ?, Assay: 25 mM sodium phosphate buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C ? mM 4-Methylumbelliferyl butyrate 4-Methylumbelliferone , Butyric Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase Ethanol 10 % v/v 100 149 % Digital Continuous Incubation: ?, Assay: 25 mM sodium phosphate buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C ? mM 4-Methylumbelliferyl butyrate 4-Methylumbelliferone , Butyric Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase Methanol 70 % v/v 100 105 % Digital Continuous Incubation: ?, Assay: 25 mM sodium phosphate buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C ? mM 4-Methylumbelliferyl butyrate 4-Methylumbelliferone , Butyric Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase Methanol 80 % v/v 100 30 % Digital Continuous Incubation: ?, Assay: 25 mM sodium phosphate buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C ? mM 4-Methylumbelliferyl butyrate 4-Methylumbelliferone , Butyric Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase Methanol 90 % v/v 100 4 % Digital Continuous Incubation: ?, Assay: 25 mM sodium phosphate buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C ? mM 4-Methylumbelliferyl butyrate 4-Methylumbelliferone , Butyric Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase Isopropanol 10 % v/v 100 213 % Digital Continuous Incubation: ?, Assay: 25 mM sodium phosphate buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C ? mM 4-Methylumbelliferyl butyrate 4-Methylumbelliferone , Butyric Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase Isopropanol 20 % v/v 100 242 % Digital Continuous Incubation: ?, Assay: 25 mM sodium phosphate buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C ? mM 4-Methylumbelliferyl butyrate 4-Methylumbelliferone , Butyric Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase Isopropanol 30 % v/v 100 266 % Digital Continuous Incubation: ?, Assay: 25 mM sodium phosphate buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C ? mM 4-Methylumbelliferyl butyrate 4-Methylumbelliferone , Butyric Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase Isopropanol 40 % v/v 100 292 % Digital Continuous Incubation: ?, Assay: 25 mM sodium phosphate buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C ? mM 4-Methylumbelliferyl butyrate 4-Methylumbelliferone , Butyric Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase Isopropanol 50 % v/v 100 353 % Digital Continuous Incubation: ?, Assay: 25 mM sodium phosphate buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C ? mM 4-Methylumbelliferyl butyrate 4-Methylumbelliferone , Butyric Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase Isopropanol 60 % v/v 100 389 % Digital Continuous Incubation: ?, Assay: 25 mM sodium phosphate buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C ? mM 4-Methylumbelliferyl butyrate 4-Methylumbelliferone , Butyric Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase Isopropanol 70 % v/v 100 428 % Digital Continuous Incubation: ?, Assay: 25 mM sodium phosphate buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C ? mM 4-Methylumbelliferyl butyrate 4-Methylumbelliferone , Butyric Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase Isopropanol 80 % v/v 100 476 % Digital Continuous Incubation: ?, Assay: 25 mM sodium phosphate buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C ? mM 4-Methylumbelliferyl butyrate 4-Methylumbelliferone , Butyric Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase Isopropanol 90 % v/v 100 472 % Digital Continuous Incubation: ?, Assay: 25 mM sodium phosphate buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C ? mM 4-Methylumbelliferyl butyrate 4-Methylumbelliferone , Butyric Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase Acetone 10 % v/v 100 104 % Digital Continuous Incubation: ?, Assay: 25 mM sodium phosphate buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C ? mM 4-Methylumbelliferyl butyrate 4-Methylumbelliferone , Butyric Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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