Cellulase (gene MaCel) from Mahella australiensis 50-1 BON

Enzyme Description

Species
Mahella australiensis 50-1 BON β†—
Extremophile
Yes "thermophilic" organism
EC Number
3.2.1.4 β†—

Sequence

Length: 426 amino acids
MNKRKMLAFMIAIVLILSSLMTSTIVVRKTEAAASSIVASHGMLKVVGNKVCDASGKPIQLKGMSLFWSQWSTPFWNADLVNNLASTWNATLIRAAMGVDEGGYISNPQVEKQKVITVVDAAINAGIYVIIDWHSHDIHTSEAVAFFGEMAQRYKNTPNVIFEIFNEPDYESWAQVKTYSTQVINTIRSTGAQNLILVGSPTWSQDVDVAANDPITGFSNIAYTLHFYAGTHKQYLRDKAITAMSKGLALFVSEWGTCDASGNGNLDLTESQRWIDFMNQYQISWANWSMNDKAETASALVVGASTTGPWPDSQLTESGKFVKAKIMENTPVPPSGGTTPPGEYTGIGLPFTHDGAGEYYWKTNKLSTDPNDWSHYINSWNLDLLEINGKDYTNVWVAQHQIAPSSDGYWYIHYKGSASSAHMEMK
Tong Zhu et al. (2020) β€” Characterization and efficient production of a thermostable, halostable and organic solvent-stable cellulase from an oil reservoir
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2020.05.021 β†—  Β· Activity - Classical
16 measurements ▼
Database ID
UniProt: F4A377 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (16 measurements)

16 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Methanol 5 % v/v 100 100 % Digital Continuous 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Methanol 10 % v/v 100 92 % Digital Continuous 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Methanol 15 % v/v 100 87 % Digital Continuous 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Methanol 20 % v/v 100 78 % Digital Continuous 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ethanol 5 % v/v 100 97 % Digital Continuous 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ethanol 10 % v/v 100 88 % Digital Continuous 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ethanol 15 % v/v 100 78 % Digital Continuous 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ethanol 20 % v/v 100 72 % Digital Continuous 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Isopropanol 5 % v/v 100 98 % Digital Continuous 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Isopropanol 10 % v/v 100 94 % Digital Continuous 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Isopropanol 15 % v/v 100 91 % Digital Continuous 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Isopropanol 20 % v/v 100 80 % Digital Continuous 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Acetone 5 % v/v 100 95 % Digital Continuous 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Acetone 10 % v/v 100 81 % Digital Continuous 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Acetone 15 % v/v 100 75 % Digital Continuous 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Acetone 20 % v/v 100 59 % Digital Continuous 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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