Protease 1147 from Cohnella sp. A01

Enzyme Description

Species
Cohnella sp. A01 β†—
Extremophile
Yes "thermophilic" organism
EC Number
3.4.- β†—

Sequence

Length: 169 amino acids
MKKVAFLLANDFEDSEMQKPYEAIRAAGHETVIIGLKKGEKLTGKNKQATYTTDASIDEAKADQFDAVVIPGGSSPENLRLNKQIQQFVKDMDSQGKPIAAICHGPQILISAGLAKGKTLTCYPPLQDDLINAGANYVDQEVVVDGHYITSRTPADEPAFIRETLNKLS
Hossein Tarrahimofrad et al. (2020) β€” Structural and biochemical characterization of a novel thermophilic Coh01147 protease
PLOS ONE  Β· doi:10.1371/journal.pone.0234958 β†—  Β· Activity - Classical
18 measurements ▼
Database ID
UniProt: A0A5J6KIY6 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (18 measurements)

18 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Glycerol 2 % v/v 100.0 117.8 % Direct Continuous 50 mM glycine-NaOH buffer 10.5 60Β°C 0.01 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %) | Uncertainty about assay conditions (buffer, pH and temperature)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 3 % v/v 100.0 86.6 % Direct Continuous 50 mM glycine-NaOH buffer 10.5 60Β°C 0.01 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %) | Uncertainty about assay conditions (buffer, pH and temperature)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Acetone 3 % v/v 100.0 90.6 % Direct Continuous 50 mM glycine-NaOH buffer 10.5 60Β°C 0.01 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %) | Uncertainty about assay conditions (buffer, pH and temperature)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Glycerol 3 % v/v 100.0 94.4 % Direct Continuous 50 mM glycine-NaOH buffer 10.5 60Β°C 0.01 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %) | Uncertainty about assay conditions (buffer, pH and temperature)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Isopropanol 3 % v/v 100.0 86.6 % Direct Continuous 50 mM glycine-NaOH buffer 10.5 60Β°C 0.01 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %) | Uncertainty about assay conditions (buffer, pH and temperature)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Ethanol 3 % v/v 100.0 86.9 % Direct Continuous 50 mM glycine-NaOH buffer 10.5 60Β°C 0.01 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %) | Uncertainty about assay conditions (buffer, pH and temperature)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Methanol 3 % v/v 100.0 83.9 % Direct Continuous 50 mM glycine-NaOH buffer 10.5 60Β°C 0.01 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %) | Uncertainty about assay conditions (buffer, pH and temperature)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 2 % v/v 100.0 122.1 % Direct Continuous 50 mM glycine-NaOH buffer 10.5 60Β°C 0.01 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %) | Uncertainty about assay conditions (buffer, pH and temperature)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Acetone 2 % v/v 100.0 93.9 % Direct Continuous 50 mM glycine-NaOH buffer 10.5 60Β°C 0.01 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %) | Uncertainty about assay conditions (buffer, pH and temperature)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Methanol 1 % v/v 100.0 120.0 % Direct Continuous 50 mM glycine-NaOH buffer 10.5 60Β°C 0.01 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %) | Uncertainty about assay conditions (buffer, pH and temperature)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Isopropanol 2 % v/v 100.0 93.6 % Direct Continuous 50 mM glycine-NaOH buffer 10.5 60Β°C 0.01 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %) | Uncertainty about assay conditions (buffer, pH and temperature)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Ethanol 2 % v/v 100.0 98.4 % Direct Continuous 50 mM glycine-NaOH buffer 10.5 60Β°C 0.01 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %) | Uncertainty about assay conditions (buffer, pH and temperature)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Methanol 2 % v/v 100.0 113.6 % Direct Continuous 50 mM glycine-NaOH buffer 10.5 60Β°C 0.01 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %) | Uncertainty about assay conditions (buffer, pH and temperature)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 1 % v/v 100.0 116.9 % Direct Continuous 50 mM glycine-NaOH buffer 10.5 60Β°C 0.01 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %) | Uncertainty about assay conditions (buffer, pH and temperature)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Acetone 1 % v/v 100.0 92.1 % Direct Continuous 50 mM glycine-NaOH buffer 10.5 60Β°C 0.01 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %) | Uncertainty about assay conditions (buffer, pH and temperature)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Glycerol 1 % v/v 100.0 126.9 % Direct Continuous 50 mM glycine-NaOH buffer 10.5 60Β°C 0.01 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %) | Uncertainty about assay conditions (buffer, pH and temperature)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Isopropanol 1 % v/v 100.0 94.8 % Direct Continuous 50 mM glycine-NaOH buffer 10.5 60Β°C 0.01 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %) | Uncertainty about assay conditions (buffer, pH and temperature)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Ethanol 1 % v/v 100.0 99.1 % Direct Continuous 50 mM glycine-NaOH buffer 10.5 60Β°C 0.01 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %) | Uncertainty about assay conditions (buffer, pH and temperature)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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