GH39 xylosidase (gene XYL21) from a saline soil sample metagenomic library

Enzyme Description

Species
Na (saline soil sample metagenomic library)
Extremophile
Yes likely halophilic organism (from saline soil) and "halo-tolerant" enzyme
EC Number
3.2.1.37 β†—

Sequence

Length: 501 amino acids
MEKIMISRDNNIPFNKHWKKCIGTGRLGLALQKEYVDHLERLQQDIGFDYIRGHGLFHEDIGIYKEVDVGGTPTPFYNFTYIDRIFDTFLQNNLRPFVELGFMPKKLASGTQTIFYWEGNVTPPAEEKKWEELIYHTVIHFVERYGVEEVLQWPFEVWNEPNLSNFWENADKQAYFQLYKITAKTIKSIHPDLNVGGPAICGGTDEWITDFLHFCHKEQVPVDFISRHAYTSKPPKKKTPDYYYQDLADPNDMLTQLTSVKKLIHDTPYPDLPFHITEYNTSYSPINPIHDTNYNAAYLGKILSHAGDIVSSFSYWTFSDVFEEFDIPRAPFHGGFGLMALHAIRKPTYHLFSFFNKLGDTCLYKDDTMIVTKHRDNTISIVAWNLIHDKGENHDKTITLSIPFSSDDVFLYREITNEHHANPWRVWKQMGRPRFPSKEQITLLKSCDIPFIQTDKLMTENKLLTYQLTMAKNEVTLLHFSDIIDETNTYIGLDDSLLTSY
Zhongyuan Li et al. (2020) β€” Biochemical characterization of a novel halo/organic-solvents/final-products tolerant GH39 xylosidase from saline soil and its synergic action with xylanase
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2020.07.079 β†—  Β· Activity - Classical
42 measurements ▼
Database ID
UniParc: UPI00090C2DE3 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (42 measurements)

42 measurements β€” page 1 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent 1-Propanol 25 % v/v 100 11 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent 1-Butanol 10 % v/v 100 88 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent 1-Butanol 15 % v/v 100 76 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent 1-Butanol 20 % v/v 100 68 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent 1-Butanol 25 % v/v 100 55 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent 1-Butanol 30 % v/v 100 45 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent 1-Butanol 35 % v/v 100 38 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent 1-Propanol 5 % v/v 100 191 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent 1-Propanol 10 % v/v 100 48 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent 1-Propanol 15 % v/v 100 17 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent 1-Propanol 20 % v/v 100 14 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent 1-Butanol 5 % v/v 100 112 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent 1-Propanol 30 % v/v 100 6 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent 1-Propanol 35 % v/v 100 3 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Isoamyl Alcohol 5 % v/v 100 176 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Isoamyl Alcohol 10 % v/v 100 153 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Isoamyl Alcohol 15 % v/v 100 156 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Isoamyl Alcohol 20 % v/v 100 147 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Isoamyl Alcohol 25 % v/v 100 122 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Isoamyl Alcohol 30 % v/v 100 110 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
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Visualization : Activity β€” Classical

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Structure

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