GH39 xylosidase (gene XYL21) from a saline soil sample metagenomic library

Enzyme Description

Species
Na (saline soil sample metagenomic library)
Extremophile
Yes likely halophilic organism (from saline soil) and "halo-tolerant" enzyme
EC Number
3.2.1.37 β†—

Sequence

Length: 501 amino acids
MEKIMISRDNNIPFNKHWKKCIGTGRLGLALQKEYVDHLERLQQDIGFDYIRGHGLFHEDIGIYKEVDVGGTPTPFYNFTYIDRIFDTFLQNNLRPFVELGFMPKKLASGTQTIFYWEGNVTPPAEEKKWEELIYHTVIHFVERYGVEEVLQWPFEVWNEPNLSNFWENADKQAYFQLYKITAKTIKSIHPDLNVGGPAICGGTDEWITDFLHFCHKEQVPVDFISRHAYTSKPPKKKTPDYYYQDLADPNDMLTQLTSVKKLIHDTPYPDLPFHITEYNTSYSPINPIHDTNYNAAYLGKILSHAGDIVSSFSYWTFSDVFEEFDIPRAPFHGGFGLMALHAIRKPTYHLFSFFNKLGDTCLYKDDTMIVTKHRDNTISIVAWNLIHDKGENHDKTITLSIPFSSDDVFLYREITNEHHANPWRVWKQMGRPRFPSKEQITLLKSCDIPFIQTDKLMTENKLLTYQLTMAKNEVTLLHFSDIIDETNTYIGLDDSLLTSY
Zhongyuan Li et al. (2020) β€” Biochemical characterization of a novel halo/organic-solvents/final-products tolerant GH39 xylosidase from saline soil and its synergic action with xylanase
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2020.07.079 β†—  Β· Activity - Classical
42 measurements ▼
Database ID
UniParc: UPI00090C2DE3 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (42 measurements)

42 measurements β€” page 2 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Isoamyl Alcohol 35 % v/v 100 97 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Isopropanol 25 % v/v 100 9 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Methanol 10 % v/v 100 172 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Methanol 15 % v/v 100 147 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Methanol 20 % v/v 100 81 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Methanol 25 % v/v 100 30 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Methanol 30 % v/v 100 17 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Methanol 35 % v/v 100 15 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Isopropanol 5 % v/v 100 178 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Isopropanol 10 % v/v 100 166 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Isopropanol 15 % v/v 100 77 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Isopropanol 20 % v/v 100 17 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Methanol 5 % v/v 100 165 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Isopropanol 30 % v/v 100 5 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Isopropanol 35 % v/v 100 7 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Ethanol 5 % v/v 100 175 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Ethanol 10 % v/v 100 168 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Ethanol 15 % v/v 100 105 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Ethanol 20 % v/v 100 30 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Ethanol 25 % v/v 100 15 % Digital Continuous 200 mM citric acid–Naβ‚‚HPOβ‚„ buffer 5.5 45Β°C 2 mM Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %) | Uncertainty about pH and buffer
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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