Leucine aminopeptidase from Aquifex aeolicus

Enzyme Description

Species
Aquifex aeolicus β†—
Extremophile
Yes "hyperthermophilic" organism
EC Number
3.4.11.1 β†—

Sequence

Length: 493 amino acids
MKEMEVRAFKDDIKKYKGKSVAVFVYEGDLKPLARLSKGTSNKAKRVAELENFKGKEGEILKVPTLGTSVDFVYIVGLGKKEKVGEDTYRRASANLVKRMRRDKVESTVVVIPRRGDVSKEITKAITEGAILGNYRFDKYKSKKEDEKFEIKEVLINRGDEEGIRLGKIFAEAQNYARNLVNEPGNVINPITLAEEAKKLAEEFGLECKVYDEKQIQEMGMMALYSVGKGSATPPRFIHLIYKPSGKPKEKIALVGKGLTFDSGGLNIKPGDYMRTMKMDKSGACAVLGIMRAIAQLKPDVEVHGLIGAAENMPDGNAYRPDDVIKAKNGKYIEIDNTDAEGRVTLADVLSYASELKPDKIIDMATLTGACMVALGEYTAGLFTNAPDFAEEIKKTAKRTGERVWELPMDDERLRKKIKNTVADVLNTGGRYGGAITAAMFLEEFVGEGIKWVHLDIAGPAWSKEEYGYYTKGGTGFGVRTCLEYIMKVSSNV
Anisur Rahman Khan et al. (2000) β€” Characterization of a solvent resistant and thermostable aminopeptidase from the hyperthermophillic bacterium, Aquifex aeolicus
Enzyme and Microbial Technology  Β· doi:10.1016/s0141-0229(00)00194-0 β†—  Β· Activity - Classical Activity - Michaelis-Menten
69 measurements ▼
Database ID
UniProt: O67868 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (69 measurements)

69 measurements β€” page 2 of 4
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent Isopropanol 5 % v/v 100.0 62.0 % Direct Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent Isopropanol 10 % v/v 100.0 61.0 % Direct Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent Isopropanol 20 % v/v 100.0 35.0 % Direct Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent Isopropanol 30 % v/v 100.0 16.0 % Direct Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent Isopropanol 40 % v/v 100.0 3.0 % Direct Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent Isopropanol 50 % v/v 100.0 0.0 % Direct Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent 1-Propanol 5 % v/v 100.0 55.0 % Direct Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent 1-Propanol 10 % v/v 100.0 51.0 % Direct Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent 1-Propanol 20 % v/v 100.0 20.0 % Direct Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent 1-Propanol 30 % v/v 100.0 6.0 % Direct Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent 1-Propanol 40 % v/v 100.0 1.0 % Direct Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent 1-Propanol 50 % v/v 100.0 0.0 % Direct Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent Tetrahydrofuran (THF) 30 % v/v 100.0 9.0 % Direct Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent Methanol 5 % v/v 100.0 100.0 % Direct Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent Methanol 20 % v/v 100.0 100.0 % Direct Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent Methanol 30 % v/v 100.0 100.0 % Direct Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent Methanol 40 % v/v 100.0 62.0 % Direct Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent Methanol 50 % v/v 100.0 47.0 % Direct Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent Methanol 60 % v/v 100.0 29.0 % Digital Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent Methanol 70 % v/v 100.0 16.0 % Digital Continuous 50 mM Tris-HCl 8 45Β°C ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Classical aqueous control (in %)
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity β€” Michaelis-Menten

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Structure

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