Esterase (gene EstSHJ2) from Chromohalobacter canadensis

Enzyme Description

Species
Chromohalobacter canadensis β†—
Extremophile
Yes "halophilic" organism
EC Number
3.1.1.1 β†—

Sequence

Length: 213 amino acids
MLVAPVANILYLHGFNSGAASPKARLIAATCERLASEDVAPPRCVTPQLPHRPDEALACAERELHALGNNTLLIGSSMGGFLATCLAERFGVRAVLINPAVRPARLVTQWMGAPLENPYTHERFTIDERHGEALEAMTPAAVIDPGRYQVLLGSADETLNCRDALTFYAGASMLVHPSGDHGFSALADYLPAIFAHGGWTLPARSYTRSDEST
Mou Wang et al. (2020) β€” Characterization of a novel halotolerant esterase from Chromohalobacter canadensis isolated from salt well mine
3 Biotech  Β· doi:10.1007/s13205-020-02420-0 β†—  Β· Activity - Classical
20 measurements ▼
Database ID
UniProt: A0A2I4SAV9 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (20 measurements)

20 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 10 % v/v 100 95 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30 % v/v 100 56 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100 93 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 30 % v/v 100 58 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 10 % v/v 100 102 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethyl Acetate 30 % v/v 100 101 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethyl Acetate 10 % v/v 100 105 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent 1-Propanol 30 % v/v 100 97 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent 1-Propanol 10 % v/v 100 103 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 30 % v/v 100 64 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Toluene 10 % v/v 100 107 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetone 30 % v/v 100 78 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetone 10 % v/v 100 103 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent n-Hexane 30 % v/v 100 156 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent n-Hexane 10 % v/v 100 110 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetonitrile 30 % v/v 100 61 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetonitrile 10 % v/v 100 98 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Benzene 30 % v/v 100 126 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Benzene 10 % v/v 100 106 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Toluene 30 % v/v 100 135 % Direct Continuous 35 mM Tris-HCl buffer, 20% isopropanol 8 37Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %) | No completely clear it is an activity assay (might be an incubation)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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