DyP-type peroxidase from Streptomyces albidoflavus BSII#1

Enzyme Description

Species
Streptomyces albidoflavus BSII#1 β†—
Extremophile
No
EC Number
1.11.1.17 β†—

Sequence

Length: 414 amino acids
MADTPLQRPVTRRRLLGTAGATGLALGVTGAAAGYTAAAPSETVTPLTEVGSRREMFHVKHQPGITTPLHARGHLIAFDLAPGAGRKEAAALLRRWSQTADRLMAGEPAPAGGHDTGIALDAGPSALTVTFGFGHSFFARTGLEKQRPAALDPLPEFSSDRLDPRRSNGDLWVQIGADDALVAFHALRAVQKDAGQAARVRWQMDGFNRSPGATEKPMTTRNLMGQIDGTNNPVPSDEDFATRVRVPADGDPAWMAGGSYVVFRRIRMLLDDWEKLSVAGQEAVMGRRKADGAPLTGGTETTEPDLERVGEDGDLVIPLNAHARITRPDQNGGAAMLRRPFSYHDGIDEEGVPDAGLLFLCWQADPLRGFVPVQRKLDRGDALSAFIRHEASGLYAVPGGARKGGYVGQELLEG
Amos Musengi et al. (2020) β€” Production and characterisation of a novel actinobacterial DyP-type peroxidase and its application in coupling of phenolic monomers
Enzyme and Microbial Technology  Β· doi:10.1016/j.enzmictec.2020.109654 β†—  Β· Activity - Classical
20 measurements ▼
Database ID
UniParc: UPI0003C2D29C β†—
Sequence Annotation
Explicit - Provided
Protein Source
Purified, bacterium Streptomyces sp. BSII#1

Experimental Data (20 measurements)

20 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent 1-Propanol 30 % v/v 100 36 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent Acetonitrile 40 % v/v 100 6 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 40 % v/v 100 8 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent Ethanol 40 % v/v 100 30 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent Methanol 40 % v/v 100 43 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent 1-Propanol 40 % v/v 100 44 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent Acetonitrile 30 % v/v 100 6 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30 % v/v 100 14 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent Ethanol 30 % v/v 100 36 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent Methanol 30 % v/v 100 46 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent 1-Propanol 10 % v/v 100 68 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent Acetonitrile 20 % v/v 100 6 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20 % v/v 100 26 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent Ethanol 20 % v/v 100 55 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent Methanol 20 % v/v 100 47 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent 1-Propanol 20 % v/v 100 45 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent Acetonitrile 10 % v/v 100 12 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100 44 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent Ethanol 10 % v/v 100 58 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.4-DCP oxidized product absorbance measurement, 510 nm) in the presence of organic solvent Methanol 10 % v/v 100 61 % Digital Continuous 100 mM Tris–HCl buffer 8 25Β°C ? mM 2,4-Dichlorophenol, ? mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) 2,4-dichlorophenoxy radical , H2O β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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