Ξ²-glucosidase from Alicyclobacillus herbarius

Enzyme Description

Species
Alicyclobacillus herbarius β†—
Extremophile
Yes "acidophilic" organism
EC Number
3.2.1.21 β†—

Sequence

Length: 452 amino acids
MTREFISFPQDFLFGTATASYQIEGAVHEDGRGESIWDRFSHTPGKVYQGHTGDVACDHYHRYREDVALMKELGIPAYRFSIAWPRIFPEKGMKNEAGLDFYRRLLEALHEADIRSFVTLYHWDLPQWLQDRGGWANRDTAEYFAEYASLIYERLGDGIDAFITHNEPWCAAFLGHGFGVHAPGHTDWREAFQAAHHILYSHGLAVQAHRASSHKGQIGITLNFTWVDAATDSATDQAAAEVSHAFNNRWFLEPVAGRGYPQEFQQLVEQRIGQFDFVRQGDLAVIAEPIDFLGINFYTRSVVAANPDDALFGLRTLEAPADNRTEMGWEIHPDSLYRLLTWVQSVTGQLPLYITENGAAFADEPVNGRVEDVRRIHYIADHLEAAKRFVDAGGPLKGYFLWSFMDNFEWALGYSKRFGMVYVDYESQQRLVKDSGRWFSEQIAAHKGQVRA
Lidia Delgado et al. (2020) β€” Release of Soybean Isoflavones by Using a Ξ²-Glucosidase from Alicyclobacillus herbarius
ChemBioChem  Β· doi:10.1002/cbic.202000688 β†—  Β· Activity - Classical Stability - Incubation
96 measurements ▼
Database ID
UniProt: A0A8I3B065 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (96 measurements)

96 measurements β€” page 1 of 5
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Methanol 20 % v/v 100 59 % Digital Continuous 50 mM HEPES buffer 7.4 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Methanol 10 % v/v 100 124 % Digital Continuous 50 mM HEPES buffer 7.4 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Tetrahydrofuran (THF) 20 % v/v 100 78 % Digital Continuous 50 mM HEPES buffer 7.4 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Tetrahydrofuran (THF) 10 % v/v 100 106 % Digital Continuous 50 mM HEPES buffer 7.4 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Acetonitrile 20 % v/v 100 53 % Digital Continuous 50 mM HEPES buffer 7.4 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Acetonitrile 10 % v/v 100 70 % Digital Continuous 50 mM HEPES buffer 7.4 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20 % v/v 100 117 % Digital Continuous 50 mM HEPES buffer 7.4 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100 139 % Digital Continuous 50 mM HEPES buffer 7.4 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Isopropanol 20 % v/v 100 143 % Digital Continuous 50 mM HEPES buffer 7.4 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Isopropanol 10 % v/v 100 173 % Digital Continuous 50 mM HEPES buffer 7.4 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Ethanol 20 % v/v 100 156 % Digital Continuous 50 mM HEPES buffer 7.4 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Ethanol 10 % v/v 100 174 % Digital Continuous 50 mM HEPES buffer 7.4 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Stability - Incubation Activity after incubation (48 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Acetone 10 % v/v 100 81 % Digital Continuous Incubation: ?, Assay: 50 mM HEPES buffer Incubation: ?, Assay: 7.4 Incubation: 25Β°C, Assay: 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100 66 % Digital Continuous Incubation: ?, Assay: 50 mM HEPES buffer Incubation: ?, Assay: 7.4 Incubation: 25Β°C, Assay: 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent
Stability - Incubation Activity after incubation (24 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Acetone 10 % v/v 100 81 % Digital Continuous Incubation: ?, Assay: 50 mM HEPES buffer Incubation: ?, Assay: 7.4 Incubation: 25Β°C, Assay: 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Acetone 10 % v/v 100 88 % Digital Continuous Incubation: ?, Assay: 50 mM HEPES buffer Incubation: ?, Assay: 7.4 Incubation: 25Β°C, Assay: 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Acetone 10 % v/v 100 93 % Digital Continuous Incubation: ?, Assay: 50 mM HEPES buffer Incubation: ?, Assay: 7.4 Incubation: 25Β°C, Assay: 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Acetone 10 % v/v 100 87 % Digital Continuous Incubation: ?, Assay: 50 mM HEPES buffer Incubation: ?, Assay: 7.4 Incubation: 25Β°C, Assay: 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Acetone 10 % v/v 100 73 % Digital Continuous Incubation: ?, Assay: 50 mM HEPES buffer Incubation: ?, Assay: 7.4 Incubation: 25Β°C, Assay: 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent
Stability - Incubation Activity after incubation (5 days at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20 % v/v 100 14 % Digital Continuous Incubation: ?, Assay: 50 mM HEPES buffer Incubation: ?, Assay: 7.4 Incubation: 25Β°C, Assay: 25Β°C 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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