β-glucosidase from Alicyclobacillus herbarius

Enzyme Description

Species

Alicyclobacillus herbarius ↗

Extremophile

Yes "acidophilic" organism

EC Number

3.2.1.21 ↗

Sequence

Length: 452 amino acids

MTREFISFPQDFLFGTATASYQIEGAVHEDGRGESIWDRFSHTPGKVYQGHTGDVACDHYHRYREDVALMKELGIPAYRFSIAWPRIFPEKGMKNEAGLDFYRRLLEALHEADIRSFVTLYHWDLPQWLQDRGGWANRDTAEYFAEYASLIYERLGDGIDAFITHNEPWCAAFLGHGFGVHAPGHTDWREAFQAAHHILYSHGLAVQAHRASSHKGQIGITLNFTWVDAATDSATDQAAAEVSHAFNNRWFLEPVAGRGYPQEFQQLVEQRIGQFDFVRQGDLAVIAEPIDFLGINFYTRSVVAANPDDALFGLRTLEAPADNRTEMGWEIHPDSLYRLLTWVQSVTGQLPLYITENGAAFADEPVNGRVEDVRRIHYIADHLEAAKRFVDAGGPLKGYFLWSFMDNFEWALGYSKRFGMVYVDYESQQRLVKDSGRWFSEQIAAHKGQVRA

Lidia Delgado et al. (2020) — Release of Soybean Isoflavones by Using a β-Glucosidase from Alicyclobacillus herbarius

ChemBioChem · doi:10.1002/cbic.202000688 ↗ · Activity - Classical Stability - Incubation

96 measurements ▼

Database ID

UniProt: A0A8I3B065 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (96 measurements)

96 measurements — page 5 of 5

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	20 % v/v	100	81	%	Digital	Continuous	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	20 % v/v	100	96	%	Digital	Continuous	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10 % v/v	100	12	%	Digital	Continuous	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10 % v/v	100	82	%	Digital	Continuous	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10 % v/v	100	73	%	Digital	Continuous	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10 % v/v	100	81	%	Digital	Continuous	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	20 % v/v	100	15	%	Digital	Continuous	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10 % v/v	100	87	%	Digital	Continuous	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10 % v/v	100	72	%	Digital	Continuous	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20 % v/v	100	17	%	Digital	Continuous	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20 % v/v	100	85	%	Digital	Continuous	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20 % v/v	100	83	%	Digital	Continuous	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20 % v/v	100	87	%	Digital	Continuous	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20 % v/v	100	91	%	Digital	Continuous	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20 % v/v	100	88	%	Digital	Continuous	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20 % v/v	100	93	%	Digital	Continuous	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Methanol

20 % v/v

100

Digital

Continuous

50 mM HEPES buffer

7.4

25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Methanol

10 % v/v

100

124

Digital

Continuous

50 mM HEPES buffer

7.4

25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

20 % v/v

100

Digital

Continuous

50 mM HEPES buffer

7.4

25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

10 % v/v

100

106

Digital

Continuous

50 mM HEPES buffer

7.4

25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Acetonitrile

20 % v/v

100

Digital

Continuous

50 mM HEPES buffer

7.4

25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Acetonitrile

10 % v/v

100

Digital

Continuous

50 mM HEPES buffer

7.4

25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100

117

Digital

Continuous

50 mM HEPES buffer

7.4

25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

139

Digital

Continuous

50 mM HEPES buffer

7.4

25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Isopropanol

20 % v/v

100

143

Digital

Continuous

50 mM HEPES buffer

7.4

25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Isopropanol

10 % v/v

100

173

Digital

Continuous

50 mM HEPES buffer

7.4

25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

156

Digital

Continuous

50 mM HEPES buffer

7.4

25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

174

Digital

Continuous

50 mM HEPES buffer

7.4

25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Classical aqueous control (in %)

Stability - Incubation

Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Acetone

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Acetone

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Acetone

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Acetone

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Acetone

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Acetone

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Acetone

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Acetone

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Acetone

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Acetone

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Acetone

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Acetone

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Acetone

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Acetone

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Methanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

108

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Methanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Methanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Methanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Methanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Methanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Methanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Methanol

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Methanol

10 % v/v

100

104

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Methanol

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Methanol

10 % v/v

100

109

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Methanol

10 % v/v

100

102

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Methanol

10 % v/v

100

101

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Methanol

10 % v/v

100

107

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Isopropanol

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Isopropanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Isopropanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Isopropanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Isopropanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Isopropanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Isopropanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Isopropanol

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Isopropanol

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Isopropanol

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Isopropanol

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Isopropanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Isopropanol

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Isopropanol

10 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

Digital

Continuous

Incubation: ?, Assay: 50 mM HEPES buffer

Incubation: ?, Assay: 7.4

Incubation: 25°C, Assay: 25°C

10 mM p-Nitrophenyl β-D-glucopyranoside

D-Glucose, p-Nitrophenol

—

Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in organic solvent

‹ Prev

1 2 3 4 5

Next ›

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate · Scroll to zoom · Right-click drag to pan · Powered by Mol*

β-glucosidase from Alicyclobacillus herbarius

Enzyme Description

Sequence

Experimental Data (96 measurements)

Visualization : Activity — Classical

Visualization : Stability — Incubation

Structure

Loading…