Polysaccharide lyase (gene MtAl138) from Microbulbifer thermotolerans DAU221

Enzyme Description

Species
Microbulbifer thermotolerans DAU221 β†—
Extremophile
No but "thermotolerant" organism
EC Number
4.2.2.3 β†—

Sequence

Length: 744 amino acids
MKNSKYTPATTISCAALFSLCMAHAHASIQNSSFENGWDSWTSSGSSAISDIAYSGSKSAKINGSNGYFEQEVTVDANSNYRLTVYVFGEGSVGVESNGNVYSKEVSNSDWELVTVEFNTGSSQTITVFGAYTDGDVRFDDFALEKISSSSTSIENAGFENDWDNWSSSGASAISDISYKGSKSAKVNGSNGRFEQNVAVQPYTDYELTAYILGDGILGVEANGSTYEKSVSNSNWEAETVQFNSGSAQTITIFGEYDGSDGRFDEFSLEEVSSSGSCASTSSLTIVDAYDDGTNDGHGPENVLDGSLADESRWSSQGVKWITLDLGTEKTVEGVDIAWYNGDQRKNYFYIETSIDNINWTTVLNGGQSSGTSSDMEFYDVADTTARYVRLTGSGNSYDSWNSILEFDVIGCGDGSEESSSSSSSSSSSSSSSSSSSSSSSSSSGGGSSSSSSSSSSSGGFSNLDPSLPPGSNFELEDWYLSIPTDDDGNGKADSIKEGALSGGYENSSYFYTASDGGMVFRCPIDGYKTSSGTSYTRTELREMLRRGDTSIDTQGVNKNNWVFGSAPAEAREAAGGVDGVLRATLAVNHVTTTGDSSQVGRVIIGQIHANDDEPLRIYYRKLPDNTKGSIYIAHEPNGGSDQWYEMIGSRSDDASDPADGIALNEIFSYEVKVVGNTLTVTISRDGKEDVVQEVDMSDSGYDTEDQYQYFKAGVYNQNNTGDDDDYVQATFYSLENSHDGYDY
Hae-Rin Jeong et al. (2021) β€” Characterization of an organic solvent-tolerant polysaccharide lyase from Microbulbifer thermotolerans DAU221
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2020.12.138 β†—  Β· Activity - Classical
22 measurements ▼
Database ID
UniProt: A0A7D5KCK1 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (22 measurements)

22 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20 % v/v 100 9 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent n-Hexane 20 % v/v 100 205 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent Toluene 20 % v/v 100 156 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent Benzene 20 % v/v 100 358 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent Isoamyl Alcohol 20 % v/v 100 78 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent 1-Butanol 20 % v/v 100 33 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent Isopropanol 20 % v/v 100 79 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent Acetone 20 % v/v 100 0 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent Ethanol 20 % v/v 100 25 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent Acetonitrile 20 % v/v 100 14 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent Methanol 20 % v/v 100 114 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100 12 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent n-Hexane 10 % v/v 100 169 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent Toluene 10 % v/v 100 118 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent Benzene 10 % v/v 100 130 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent Isoamyl Alcohol 10 % v/v 100 79 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent 1-Butanol 10 % v/v 100 16 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent Isopropanol 10 % v/v 100 136 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent Acetone 10 % v/v 100 3 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (Ξ”4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent Ethanol 10 % v/v 100 38 % Direct Continuous 20 mM sodium phosphate buffer 7.4 45Β°C 0.2% (w/v) Alginate Ξ”4,5-unsaturated oligoalginate β€” β€” Classical aqueous control (in %)
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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