GH5 endoglucanase (gene celM) from a Tattapan extreme-temperature hot spring metagenomic library

Enzyme Description

Species
Na (Tattapan extreme-temperature hot spring metagenomic library)
Extremophile
Yes likely thermophilic organism (extreme-temperature hot spring), "thermo-halo-tolerant" enzyme
EC Number
3.2.1.4 β†—

Sequence

Length: 304 amino acids
MQRGVNLGNALEAPYEGAWGVILREEYFRLIREAGFDTVRIPIRWSAHANPVPPYTIDERFFRRVDWAIDQALLHGLTVIINVHHYDELMTNPEEHRERFLALWDQIARRYAARPATLLFELLNEPHARLTAPVWNQLLAEALQVVRQTNPTRWVVIGPTQWNHVRSLPSLELPKEDPHIIVTFHYYEPFPFTHQGASWVPGSMVWLGTKWEGREEERQTIVQDLDFAVLWAREQNRPLFLGEFGAYSRADLPSRVRWTEFVARQAEARGIPWCYWEFCSGFGIYDPRTQRWRDQLLWALIPRP
Namrata Joshi et al. (2021) β€” Biochemical characterization of a novel thermo-halo-tolerant GH5 endoglucanase from a thermal spring metagenome
Biotechnology and Bioengineering  Β· doi:10.1002/bit.27668 β†—  Β· Activity - Classical
6 measurements ▼
Database ID
UniParc: UPI001AF0C561 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (6 measurements)

6 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5 % v/v 100 83 % Digital Continuous 20 mM sodium phosphate buffer 6 80Β°C 0.005 CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Acetonitrile 5 % v/v 100 78 % Digital Continuous 20 mM sodium phosphate buffer 6 80Β°C 0.005 CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ethanol 5 % v/v 100 68 % Digital Continuous 20 mM sodium phosphate buffer 6 80Β°C 0.005 CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent 1-Propanol 5 % v/v 100 66 % Digital Continuous 20 mM sodium phosphate buffer 6 80Β°C 0.005 CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Methanol 5 % v/v 100 50 % Digital Continuous 20 mM sodium phosphate buffer 6 80Β°C 0.005 CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent 1-Butanol 5 % v/v 100 26 % Digital Continuous 20 mM sodium phosphate buffer 6 80Β°C 0.005 CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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