Serine protease SAPGB from Gracilibacillus boraciitolerans strain LO15

Enzyme Description

Species
Gracilibacillus boraciitolerans LO15 β†—
Extremophile
No but "moderately halotolerant" organism
EC Number
3.4.- β†—

Sequence

Length: 276 amino acids
EQTVPWRIDYIGKAAAHQKGWFGKGIRVGIIDSGIRPHKDLKVSGGINILDNSENYFDGYGHGTWYAGIIGALDNSYGLLGVASDADLYSIKVKKNDGKGLISDVVTGIEWAIENKMNIVNFSLQTNVETDILRKAIKKANENDILLVASSGNMGGTKKDDTVTYPAAYDEVISVAAINKNGERYVHSSTGKRIDISAPGELVYTTVQSGLYFTLNGTSMSAPHDSGVAALVLQNNPKLNIEEIRKILVKSTKPLGNPHLYGKGLIDVQKAIKFSN
Akli Ouelhadj et al. (2020) β€” Identification and homology modeling of a new biotechnologically compatible serine alkaline protease from moderately halotolerant Gracilibacillus boraciitolerans strain LO15
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2020.07.266 β†—  Β· Activity - Classical
10 measurements ▼
Database ID
UniProt: A0A7L9CQD4 β†—
Sequence Annotation
Explicit - Provided (first 110 residues from UniProt sequence absent from the mature protein)
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3) pLys

Experimental Data (10 measurements)

10 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent n-Decane 25 % v/v 100 79 % Digital Continuous 83 mM glycine-NaOH buffer 10 60Β°C 0.008 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent n-Octane 25 % v/v 100 101 % Digital Continuous 83 mM glycine-NaOH buffer 10 60Β°C 0.008 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent n-Hexane 25 % v/v 100 190 % Digital Continuous 83 mM glycine-NaOH buffer 10 60Β°C 0.008 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Toluene 25 % v/v 100 70 % Digital Continuous 83 mM glycine-NaOH buffer 10 60Β°C 0.008 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Chloroform 25 % v/v 100 175 % Digital Continuous 83 mM glycine-NaOH buffer 10 60Β°C 0.008 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent 1-Butanol 25 % v/v 100 88 % Digital Continuous 83 mM glycine-NaOH buffer 10 60Β°C 0.008 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Isopropanol 25 % v/v 100 115 % Digital Continuous 83 mM glycine-NaOH buffer 10 60Β°C 0.008 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Acetonitrile 25 % v/v 100 0 % Digital Continuous 83 mM glycine-NaOH buffer 10 60Β°C 0.008 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Methanol 25 % v/v 100 103 % Digital Continuous 83 mM glycine-NaOH buffer 10 60Β°C 0.008 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 25 % v/v 100 129 % Digital Continuous 83 mM glycine-NaOH buffer 10 60Β°C 0.008 Casein free amino acids , Peptides β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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