Egg-white lysozyme from Gallus gallus (chicken)

Enzyme Description

Species

Extremophile

EC Number

3.2.1.17 ↗

Sequence

Length: 129 amino acids

KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL

Bing Lai et al. (2000) — Organic cosolvents and hen egg white lysozyme folding

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology · doi:10.1016/s0167-4838(00)00189-8 ↗ · Stability - Tm

10 measurements ▼

Database ID

UniProt: P00698 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Commercially purchased

Experimental Data (10 measurements)

10 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	2,2,2-Trifluoroethanol (TFE)	5 % v/v	74.3	62.8	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	Ethanol	5 % v/v	74.3	68.5	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	Dimethyl Sulfoxide (DMSO)	5 % v/v	74.3	72.8	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	Acetonitrile	5 % v/v	74.3	66.1	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	Glycerol	5 % v/v	74.3	75.8	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	2,2,2-Trifluoroethanol (TFE)	15 % v/v	74.3	45.7	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	Ethanol	15 % v/v	74.3	61.5	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	Dimethyl Sulfoxide (DMSO)	15 % v/v	74.3	70.4	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	Acetonitrile	15 % v/v	74.3	57.0	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	Glycerol	15 % v/v	74.3	77.0	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)

Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	2,2,2-Trifluoroethanol (TFE)	5 % v/v	74.3	62.8	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	Ethanol	5 % v/v	74.3	68.5	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	Dimethyl Sulfoxide (DMSO)	5 % v/v	74.3	72.8	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	Acetonitrile	5 % v/v	74.3	66.1	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	Glycerol	5 % v/v	74.3	75.8	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	2,2,2-Trifluoroethanol (TFE)	15 % v/v	74.3	45.7	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	Ethanol	15 % v/v	74.3	61.5	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	Dimethyl Sulfoxide (DMSO)	15 % v/v	74.3	70.4	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	Acetonitrile	15 % v/v	74.3	57.0	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm (in °C) measured in the presence of organic solvent by DSC	Glycerol	15 % v/v	74.3	77.0	°C	Direct	Continuous	40 mM phosphate buffer	6.9	—	—	—	—	—	Classical aqueous control (in °C)

Visualization : Stability — T_m

Bing Lai et al. (2000)

One bar per measurement. Colour = solvent, shade = solvent volume.

Timur Magsumov et al. (2020) — Comparative study of the protein denaturing ability of different organic cosolvents

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.05.260 ↗ · Stability - deltaHTm Stability - Tm

92 measurements ▼

Database ID

UniProt: P00698 ↗

Sequence Annotation

Inferred - from protein name (18 first residues from UniProt sequence absent from the mature protein)

Protein Source

Commercially purchased

Experimental Data (92 measurements)

92 measurements — page 4 of 5

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetone	0.15 Molar fraction	77.5	50.0	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetone	0.1 Molar fraction	77.5	56.3	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetone	0.05 Molar fraction	77.5	65.4	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetone	0.01 Molar fraction	77.5	75.0	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetonitrile	0.25 Molar fraction	77.5	41.0	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetonitrile	0.2 Molar fraction	77.5	43.4	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetonitrile	0.15 Molar fraction	77.5	48.2	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetonitrile	0.1 Molar fraction	77.5	56.8	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetonitrile	0.05 Molar fraction	77.5	66.7	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	tert-Butanol	0.1 Molar fraction	77.5	46.6	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Dimethyl Sulfoxide (DMSO)	0.3 Molar fraction	77.5	51.3	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Dimethyl Sulfoxide (DMSO)	0.25 Molar fraction	77.5	57.1	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Dimethyl Sulfoxide (DMSO)	0.2 Molar fraction	77.5	63.4	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Dimethyl Sulfoxide (DMSO)	0.1 Molar fraction	77.5	70.8	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Dimethyl Sulfoxide (DMSO)	0.05 Molar fraction	77.5	74.1	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Tetrahydrofuran (THF)	0.08 Molar fraction	77.5	38.3	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Tetrahydrofuran (THF)	0.05 Molar fraction	77.5	50.5	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Tetrahydrofuran (THF)	0.03 Molar fraction	77.5	61.4	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Tetrahydrofuran (THF)	0.01 Molar fraction	77.5	72.8	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	tert-Butanol	0.2 Molar fraction	77.5	38.5	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH

Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Acetone	0.25 Molar fraction	494.0	329.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Methanol	0.1 Molar fraction	494.0	527.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Methanol	0.05 Molar fraction	494.0	503.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Dimethylformamide (DMF)	0.25 Molar fraction	494.0	382.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Dimethylformamide (DMF)	0.2 Molar fraction	494.0	412.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Dimethylformamide (DMF)	0.15 Molar fraction	494.0	430.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Dimethylformamide (DMF)	0.1 Molar fraction	494.0	455.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Dimethylformamide (DMF)	0.05 Molar fraction	494.0	496.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	1,4-Dioxane	0.15 Molar fraction	494.0	286.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	1,4-Dioxane	0.1 Molar fraction	494.0	382.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	1,4-Dioxane	0.05 Molar fraction	494.0	427.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	1,4-Dioxane	0.01 Molar fraction	494.0	479.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Methanol	0.15 Molar fraction	494.0	499.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Acetone	0.2 Molar fraction	494.0	402.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Acetone	0.15 Molar fraction	494.0	460.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Acetone	0.1 Molar fraction	494.0	495.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Acetone	0.05 Molar fraction	494.0	504.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Acetone	0.01 Molar fraction	494.0	495.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Acetonitrile	0.25 Molar fraction	494.0	306.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Acetonitrile	0.2 Molar fraction	494.0	340.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Acetonitrile	0.15 Molar fraction	494.0	390.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Acetonitrile	0.1 Molar fraction	494.0	460.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Acetonitrile	0.05 Molar fraction	494.0	505.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	tert-Butanol	0.1 Molar fraction	494.0	325.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Dimethyl Sulfoxide (DMSO)	0.3 Molar fraction	494.0	415.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Dimethyl Sulfoxide (DMSO)	0.25 Molar fraction	494.0	476.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Dimethyl Sulfoxide (DMSO)	0.2 Molar fraction	494.0	508.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Dimethyl Sulfoxide (DMSO)	0.1 Molar fraction	494.0	569.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Dimethyl Sulfoxide (DMSO)	0.05 Molar fraction	494.0	539.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Tetrahydrofuran (THF)	0.08 Molar fraction	494.0	334.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Tetrahydrofuran (THF)	0.05 Molar fraction	494.0	417.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Tetrahydrofuran (THF)	0.03 Molar fraction	494.0	441.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Tetrahydrofuran (THF)	0.01 Molar fraction	494.0	501.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	tert-Butanol	0.2 Molar fraction	494.0	280.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	tert-Butanol	0.15 Molar fraction	494.0	290.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Acetonitrile	0.01 Molar fraction	494.0	495.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	tert-Butanol	0.05 Molar fraction	494.0	430.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	tert-Butanol	0.01 Molar fraction	494.0	512.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Isopropanol	0.2 Molar fraction	494.0	343.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Isopropanol	0.15 Molar fraction	494.0	390.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Isopropanol	0.1 Molar fraction	494.0	435.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Isopropanol	0.05 Molar fraction	494.0	506.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Isopropanol	0.01 Molar fraction	494.0	498.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Methanol	0.4 Molar fraction	494.0	404.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Methanol	0.3 Molar fraction	494.0	447.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - ΔH_Tm	Calorimetric enthalpies of denaturation (in kcal/mol), measured by DSC	Methanol	0.2 Molar fraction	494.0	481.0	kJ/mol	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in kJ/mol) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetone	0.25 Molar fraction	77.5	40.0	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Methanol	0.1 Molar fraction	77.5	68.4	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Methanol	0.05 Molar fraction	77.5	73.1	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Dimethylformamide (DMF)	0.25 Molar fraction	77.5	40.4	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Dimethylformamide (DMF)	0.2 Molar fraction	77.5	46.3	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Dimethylformamide (DMF)	0.15 Molar fraction	77.5	50.5	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Dimethylformamide (DMF)	0.1 Molar fraction	77.5	56.4	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Dimethylformamide (DMF)	0.05 Molar fraction	77.5	64.6	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	1,4-Dioxane	0.15 Molar fraction	77.5	39.2	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	1,4-Dioxane	0.1 Molar fraction	77.5	49.7	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	1,4-Dioxane	0.05 Molar fraction	77.5	60.8	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	1,4-Dioxane	0.01 Molar fraction	77.5	73.6	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Methanol	0.15 Molar fraction	77.5	63.3	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetone	0.2 Molar fraction	77.5	44.2	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetone	0.15 Molar fraction	77.5	50.0	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetone	0.1 Molar fraction	77.5	56.3	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetone	0.05 Molar fraction	77.5	65.4	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetone	0.01 Molar fraction	77.5	75.0	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetonitrile	0.25 Molar fraction	77.5	41.0	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetonitrile	0.2 Molar fraction	77.5	43.4	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetonitrile	0.15 Molar fraction	77.5	48.2	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetonitrile	0.1 Molar fraction	77.5	56.8	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetonitrile	0.05 Molar fraction	77.5	66.7	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	tert-Butanol	0.1 Molar fraction	77.5	46.6	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Dimethyl Sulfoxide (DMSO)	0.3 Molar fraction	77.5	51.3	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Dimethyl Sulfoxide (DMSO)	0.25 Molar fraction	77.5	57.1	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Dimethyl Sulfoxide (DMSO)	0.2 Molar fraction	77.5	63.4	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Dimethyl Sulfoxide (DMSO)	0.1 Molar fraction	77.5	70.8	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Dimethyl Sulfoxide (DMSO)	0.05 Molar fraction	77.5	74.1	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Tetrahydrofuran (THF)	0.08 Molar fraction	77.5	38.3	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Tetrahydrofuran (THF)	0.05 Molar fraction	77.5	50.5	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Tetrahydrofuran (THF)	0.03 Molar fraction	77.5	61.4	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Tetrahydrofuran (THF)	0.01 Molar fraction	77.5	72.8	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	tert-Butanol	0.2 Molar fraction	77.5	38.5	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	tert-Butanol	0.15 Molar fraction	77.5	42.0	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Acetonitrile	0.01 Molar fraction	77.5	75.7	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	tert-Butanol	0.05 Molar fraction	77.5	55.5	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	tert-Butanol	0.01 Molar fraction	77.5	72.8	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Isopropanol	0.2 Molar fraction	77.5	39.2	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Isopropanol	0.15 Molar fraction	77.5	43.9	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Isopropanol	0.1 Molar fraction	77.5	51.1	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Isopropanol	0.05 Molar fraction	77.5	62.5	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Isopropanol	0.01 Molar fraction	77.5	74.2	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Methanol	0.4 Molar fraction	77.5	45.0	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Methanol	0.3 Molar fraction	77.5	51.8	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH
Stability - Tm	Tm measured by DSC (organic solvents in molar fraction)	Methanol	0.2 Molar fraction	77.5	60.0	°C	Direct	Continuous	Deionized water	5.5 to 6.5	—	—	—	—	—	Classical aqueous control (in °C) \| Deducted pH

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Visualization : Stability — T_m

Timur Magsumov et al. (2020)

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — ΔH_Tm

Timur Magsumov et al. (2020)

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Egg-white lysozyme from Gallus gallus (chicken)

Enzyme Description

Sequence

Experimental Data (10 measurements)

Visualization : Stability — Tm

Experimental Data (92 measurements)

Visualization : Stability — Tm

Visualization : Stability — ΔHTm

Structure

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Visualization : Stability — T_m

Visualization : Stability — T_m

Visualization : Stability — ΔH_Tm