Laccase (gene lcc1) from Kabatiella bupleuri G3

Enzyme Description

Species
Kabatiella bupleuri G3 β†—
Extremophile
Yes "psychrophilic" organism
EC Number
1.10.3.2 β†—

Sequence

Length: 645 amino acids
MHLQKLSGLCLLAFASVSWADAVPGDRYLWGADRMGKMVRRATTTSKASSTTAKASTSSVKTSSVSSASSKSSSNIVASVSSSPPASSASSASSTSSLVADAACTHGPNSRACWGNGYSIKTDFDLKHPTTGNTVTYNLEITNTTCNPDGNGDMPCYLINNQFPGPTIVADWGDKVIINVKNSLQNNGTSLHWHGVRQLSSVGSDGVGGITECPIAPGQSRTYSFLATQFGTSWYHSHYSSQYGMGVVGQVVINGPSSSNYDIDLGTFPITDLYYGLANNVNEMTLHNLQNNLPPPDGSNILVNGKGKSAKGGSYSQMSLTKGKKHRLRLINTSVDNAVRVKLDGHSFTVMSADFIPIKPLTNQGWILLAVGQRYDVVFTANQTPGNYFFRAEVATDCFSGNTGKGRALFTYSEVTAAEPTDSNEVAPTNGCTELNTVPYWTQAVDQSQYAAQVKTLKTGVAPGLTTNGQNFALWNLDTRAMMVRWDRPTIQYVFEGNTSYPDPYAVIEIPTEGTWTYWLIQMGPQNPPLPHPIHLHGHDFFVLGSGAGTFDATTAVLNFANPPRRDTSILPGLGWLLISFPANNPGIWLMHCHIAWHISEGLGVQFIEAKNSIAMPDKTTFNAECDSWRTYSKNMAWPQIDSGL
Katarzyna M. Wiβ‰ˆΓ΅niewska et al. (2021) β€” Novel Cold-Adapted Recombinant Laccase KbLcc1 from Kabatiella bupleuri G3 IBMiP as a Green Catalyst in Biotransformation
International Journal of Molecular Sciences  Β· doi:10.3390/ijms22179593 β†—  Β· Activity - Classical Stability - Incubation
48 measurements ▼
Database ID
UniProt: A0A8F5DNG6 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host yeast Pichia pastoris strain PichiaPink^TM 1

Experimental Data (48 measurements)

48 measurements β€” page 1 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Acetone 10 % v/v 100 18 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Acetone 1 % v/v 100 67 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent n-Hexane 40 % v/v 100 158 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent n-Hexane 20 % v/v 100 140 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent n-Hexane 10 % v/v 100 115 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent n-Hexane 1 % v/v 100 116 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Isopropanol 40 % v/v 100 11 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Isopropanol 20 % v/v 100 21 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Isopropanol 10 % v/v 100 66 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Isopropanol 1 % v/v 100 93 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Ethanol 40 % v/v 100 6 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Ethanol 20 % v/v 100 21 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Ethanol 10 % v/v 100 68 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Methanol 40 % v/v 100 14 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Ethanol 1 % v/v 100 99 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Acetone 20 % v/v 100 1 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Acetone 40 % v/v 100 1 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Ethyl Acetate 1 % v/v 100 74 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Ethyl Acetate 10 % v/v 100 34 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Ethyl Acetate 20 % v/v 100 23 % Digital Continuous 100 mM acetate buffer 3.5 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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