DyP-type peroxidase (gene Efeb) from Bacillus sp. BL5

Enzyme Description

Species
Bacillus sp. BL5 β†—
Extremophile
No
EC Number
1.11.1.19 β†—

Sequence

Length: 416 amino acids
MGDEKQQPEQIHRRDILKWGAMAGAAVAIGASGLGGLAPLVQTAAKSSKKDEKEDEQVVPFYGKHQAGITTAHQTYAYFAALDVTAKEKSDIITLFRNWTSLTQMLTSGKKMSAEQKNQYLPPQDTGESVDLSPSNLTVTVGFGPGFFEKDGKDRFGLKNKKPKHLAALPAMPNDNLDEKQGGGDICIQVCADDEQVAFHALRNLLNQAVGTCEVRFVNKGFLSGGKNGETPRNLFGFKDGTGNQSTKDDTLMNSIVWIQSGEPDWMTGGTYMAFRKIKMFLEIWDRSSLKDQEDTFGRRKSSGAPFGQKKETDPVKLNQIPANSHVNLAKSTGKQILRRAFSYTEGLDPKTGYMDAGLLFISFQKNPDNQFIPMLKALSAKDALNEYTQTIGSALYACPGGCKKGEYIAQRLLES
Sanam Islam Khan et al. (2021) β€” Cloning, expression and biochemical characterization of lignin-degrading DyP-type peroxidase from Bacillus sp. Strain BL5
Enzyme and Microbial Technology  Β· doi:10.1016/j.enzmictec.2021.109917 β†—  Β· Stability - Incubation
12 measurements ▼
Database ID
β€”
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Isopropanol 10 % v/v 100 62 % Digital Continuous Incubation: ?, Assay: 50 mM citrate buffer Incubation: ?, Assay: 4 Incubation: 30Β°C, Assay: 30Β°C 1 mM ABTS, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Isopropanol 25 % v/v 100 48 % Digital Continuous Incubation: ?, Assay: 50 mM citrate buffer Incubation: ?, Assay: 4 Incubation: 30Β°C, Assay: 30Β°C 1 mM ABTS, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Methanol 10 % v/v 100 128 % Digital Continuous Incubation: ?, Assay: 50 mM citrate buffer Incubation: ?, Assay: 4 Incubation: 30Β°C, Assay: 30Β°C 1 mM ABTS, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Methanol 25 % v/v 100 135 % Digital Continuous Incubation: ?, Assay: 50 mM citrate buffer Incubation: ?, Assay: 4 Incubation: 30Β°C, Assay: 30Β°C 1 mM ABTS, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Ethanol 10 % v/v 100 102 % Digital Continuous Incubation: ?, Assay: 50 mM citrate buffer Incubation: ?, Assay: 4 Incubation: 30Β°C, Assay: 30Β°C 1 mM ABTS, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Ethanol 25 % v/v 100 114 % Digital Continuous Incubation: ?, Assay: 50 mM citrate buffer Incubation: ?, Assay: 4 Incubation: 30Β°C, Assay: 30Β°C 1 mM ABTS, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase 1-Butanol 10 % v/v 100 0 % Digital Continuous Incubation: ?, Assay: 50 mM citrate buffer Incubation: ?, Assay: 4 Incubation: 30Β°C, Assay: 30Β°C 1 mM ABTS, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase 1-Butanol 25 % v/v 100 0 % Digital Continuous Incubation: ?, Assay: 50 mM citrate buffer Incubation: ?, Assay: 4 Incubation: 30Β°C, Assay: 30Β°C 1 mM ABTS, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10 % v/v 100 16 % Digital Continuous Incubation: ?, Assay: 50 mM citrate buffer Incubation: ?, Assay: 4 Incubation: 30Β°C, Assay: 30Β°C 1 mM ABTS, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 25 % v/v 100 0 % Digital Continuous Incubation: ?, Assay: 50 mM citrate buffer Incubation: ?, Assay: 4 Incubation: 30Β°C, Assay: 30Β°C 1 mM ABTS, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase 1,4-Dioxane 10 % v/v 100 36 % Digital Continuous Incubation: ?, Assay: 50 mM citrate buffer Incubation: ?, Assay: 4 Incubation: 30Β°C, Assay: 30Β°C 1 mM ABTS, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase 1,4-Dioxane 25 % v/v 100 0 % Digital Continuous Incubation: ?, Assay: 50 mM citrate buffer Incubation: ?, Assay: 4 Incubation: 30Β°C, Assay: 30Β°C 1 mM ABTS, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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