Ξ²-D-glucosidase (gene SpBGL1) from Sphingomonas sp. ATCC31555

Enzyme Description

Species
Sphingomonas sp. ATCC31555 β†—
Extremophile
No
EC Number
3.2.1.21 β†—

Sequence

Length: 801 amino acids
MRSLRRFAAIAIAASALTPFVASQPASAQTQAARAEKPLYKDASQPTEARVNDLLARMTLEEKVQQLVALWLNKGSIQDTKGEFDAAKASANFPNGLGMISRPYDRRGVTASESTAAGANAGDVNRSPEETARYVYAAQKWAVEKTRLGIPMLMHEEALHGLVAPGATSFPQSIGLASSFNPKLVEQIFSMAAKEARARGANLVLAPVVDVARDPRWGRIEETYGEDPYLVTQMGLAAIRGFQGSTLPLKSDKVFITLKHMTGHGQPENGTNIGPASLGERTLRENFFPPFEAAVKTLPVMSVMASYNEIDGIPSHANKWLLTDVLRGEWGFQGAVVSDYFAIRELMTRHHMYKDPKDAALRAIDAGVDVETPDGEAYANLVALVKEGRLSQGAIDNAVRRVLRMKFEGGLFENPYPEVKLAARTNTPEAVALARQAARESIVLLKNAQGLLPLDAKGIKRMAVIGHTHAKDTPIGGYSDVPNHVVSVLEGMQNEAKGKFAVDFAEGVRITDHRVWSKDEVKLVPDSVNDQLRAQALETAKNADVVVLVLGGNEAISREAWADEHLGDSETLDLPGPQDQLAKELFALGKPVVVILLNGRPYAVNHLAEKAPALIEGWYLGEQTGTALADVVFGRYNPGGKLPVSIARNVGQLPIYYNKKPSARRGYLFGDTTPLYPFGYGLSYTTFDISAPRLGAPVIGIADKAMVEVDVTNTGKVAGDEVVQLYVRDDLASVTRPLLELKRFERVTLKPGERRTVRFELTPDDLALWNSEMKRVVEPGTFTLSAGPNSATLKSATLTVR
Jie Zhou et al. (2022) β€” Application of Ξ²-Glucosidase in a Biphasic System for the Efficient Conversion of Polydatin to Resveratrol
Molecules  Β· doi:10.3390/molecules27051514 β†—  Β· Activity - Classical
12 measurements ▼
Database ID
NCBI: WP_019370396.1 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli JM109

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 5 % v/v 100 71 % Digital Continuous McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 10 % v/v 100 64 % Digital Continuous McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 15 % v/v 100 59 % Digital Continuous McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 20 % v/v 100 37 % Digital Continuous McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 25 % v/v 100 6 % Digital Continuous McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 30 % v/v 100 4 % Digital Continuous McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 5 % v/v 100 120 % Digital Continuous McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 10 % v/v 100 115 % Digital Continuous McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 15 % v/v 100 91 % Digital Continuous McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 20 % v/v 100 69 % Digital Continuous McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 25 % v/v 100 35 % Digital Continuous McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 30 % v/v 100 7 % Digital Continuous McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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