Ξ²-glucosidase (gene Tsbgl) from Thermoclostridium stercorarium DSM 8532

Enzyme Description

Species
Thermoclostridium stercorarium DSM 8532 β†—
Extremophile
Yes "thermophilic" organism
EC Number
3.2.1.21 β†—

Sequence

Length: 751 amino acids
ISQMTLEEKASLCSGLDAWNLKSVERLGIPSIMVSDGPHGLRKETTDPTDPGKKTTVPATCFPTAVGLASSWNRELVEKVGAALGEECQAEGIAVLLGPGTNIKRSPLCGRNFEYFSEDPYLSSEMAASHIKGVQSRGVGTSLKHFAANNQEHRRMSVDAVIDERTLREIYLASFEGAVKKAKPWTIMCSYNRVNGEYASENKFLLTDVLRNEWGFEGIVVSDWGAVNERVKGLEAGLDLEMPSSFGIGDQKIVEAVKKGELPEEVLDRTVERILNLIFKAVDNRKENAGYDREAHHKLAREAARECMVLLKNEDKILPLRKQGTIAVIGEFAKRPRYQGGGSSHVNPTIMDSPYEEIKKSAGNNADVIYAQGYFIEKDEPDEKLLEEAKQAALKADVAVIFAGLPEHYECEGYDRQHMRMPESHCTLIEEVAKVQPNVVVVLCNGSPVEMPWIDKVKGLLEAYLGGQAMGGAIADLLFGDANPSGKLAETFPKQLSDNPSYLNFPGEGDRVEYREGIFVGYRYYDKKNMEPLFPFGYGLSYTTFEYGDLKISRKEISDNETVTVSVKVKNTGDMAGKEIVQLYVRDIESSVIRPEKELKGFEKVELKPGEEKTVVFELDKRAFAYYNTGIRDWHVETGEFEILIGRSSRDIVLKDKIFVKSTVTIKKPVDRNTLVGDLLSDRVLEPVFREFIINEIKNRYLLDLLENEDKSLLSVWMRYTPLRSLANSTGGELNEEKLNRLIDTLNANIK
Cheng Zeng et al. (2023) β€” Specific and efficient hydrolysis of all outer glucosyls in protopanaxadiol type and protopanaxatriol type ginsenosides by a Ξ²-glucosidase from Thermoclostridium stercorarium
Enzyme and Microbial Technology  Β· doi:10.1016/j.enzmictec.2022.110152 β†—  Β· Activity - Classical
18 measurements ▼
Database ID
UniParc: UPI0002AD7EC8 β†—
Sequence Annotation
Explicit - Provided (7 first residues from UniProt sequence absent from the mature protein)
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (18 measurements)

18 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20 % v/v 100 78 % Digital Continuous 50 mM sodium acetate buffer 5 65Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 30 % v/v 100 0 % Digital Continuous 50 mM sodium acetate buffer 5 65Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 25 % v/v 100 0 % Digital Continuous 50 mM sodium acetate buffer 5 65Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 20 % v/v 100 15 % Digital Continuous 50 mM sodium acetate buffer 5 65Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 15 % v/v 100 35 % Digital Continuous 50 mM sodium acetate buffer 5 65Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 10 % v/v 100 46 % Digital Continuous 50 mM sodium acetate buffer 5 65Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 5 % v/v 100 68 % Digital Continuous 50 mM sodium acetate buffer 5 65Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30 % v/v 100 70 % Digital Continuous 50 mM sodium acetate buffer 5 65Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 25 % v/v 100 72 % Digital Continuous 50 mM sodium acetate buffer 5 65Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 5 % v/v 100 98 % Digital Continuous 50 mM sodium acetate buffer 5 65Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 15 % v/v 100 88 % Digital Continuous 50 mM sodium acetate buffer 5 65Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100 93 % Digital Continuous 50 mM sodium acetate buffer 5 65Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5 % v/v 100 95 % Digital Continuous 50 mM sodium acetate buffer 5 65Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 30 % v/v 100 6 % Digital Continuous 50 mM sodium acetate buffer 5 65Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 25 % v/v 100 11 % Digital Continuous 50 mM sodium acetate buffer 5 65Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 20 % v/v 100 26 % Digital Continuous 50 mM sodium acetate buffer 5 65Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 15 % v/v 100 47 % Digital Continuous 50 mM sodium acetate buffer 5 65Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 10 % v/v 100 85 % Digital Continuous 50 mM sodium acetate buffer 5 65Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate Β· Scroll to zoom Β· Right-click drag to pan Β· Powered by Mol*