Chitosinase (gene ChoK) from Bacillus sp. CK4

Enzyme Description

Species

Bacillus sp. CK4 ↗

Extremophile

Yes "thermophilic" organism

EC Number

3.2.1.132 ↗

Sequence

Length: 273 amino acids

MREADWWGKAAISLLVFTMFFTLMMSETVFAAGLNKDQKRRAEQLRRICEDGTTEMRYPYVARLDDARPSTCGPAGVTTATRDGFEVVPVYKQAVANKKLPNYLAGLRRLEKEASDDTSKLKGPASAWKSLADDKAFRAAQDGVNDQVYYQPAMERSDNAGLTTALARAVMYHTVRQRGDGDDGDSRYALIKRTPKGAGGSPKEGIDEQKCLNKFSHVRYDDLMNGANHDRRDEWRESVGRVHVLRSIANQNNYNLNGGIHVRSHEYGNGVIK

Ho-Geun YOON et al. (2001) — Thermostable Chitosanase from Bacillus sp. Strain CK4: Its Purification, Characterization, and Reaction Patterns

Bioscience, Biotechnology, and Biochemistry · doi:10.1271/bbb.65.802 ↗ · Stability - Incubation

30 measurements ▼

Database ID

UniProt: Q9RC18 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, bacterium Bacillus sp. CK4

Experimental Data (30 measurements)

30 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Acetone	10 % v/v	100	98	%	Digital	Continuous	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 60°C, Assay: 60°C	0.25% soluble Chitosan	Reducing sugars	—	Incubation: 400 rpm	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Methanol	50 % v/v	100	50	%	Digital	Continuous	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 60°C, Assay: 60°C	0.25% soluble Chitosan	Reducing sugars	—	Incubation: 400 rpm	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Methanol	40 % v/v	100	70	%	Digital	Continuous	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 60°C, Assay: 60°C	0.25% soluble Chitosan	Reducing sugars	—	Incubation: 400 rpm	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Methanol	30 % v/v	100	86	%	Digital	Continuous	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 60°C, Assay: 60°C	0.25% soluble Chitosan	Reducing sugars	—	Incubation: 400 rpm	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Methanol	20 % v/v	100	95	%	Digital	Continuous	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 60°C, Assay: 60°C	0.25% soluble Chitosan	Reducing sugars	—	Incubation: 400 rpm	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Methanol	10 % v/v	100	100	%	Digital	Continuous	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 60°C, Assay: 60°C	0.25% soluble Chitosan	Reducing sugars	—	Incubation: 400 rpm	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Ethanol	50 % v/v	100	60	%	Digital	Continuous	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 60°C, Assay: 60°C	0.25% soluble Chitosan	Reducing sugars	—	Incubation: 400 rpm	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Ethanol	40 % v/v	100	75	%	Digital	Continuous	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 60°C, Assay: 60°C	0.25% soluble Chitosan	Reducing sugars	—	Incubation: 400 rpm	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Ethanol	30 % v/v	100	88	%	Digital	Continuous	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 60°C, Assay: 60°C	0.25% soluble Chitosan	Reducing sugars	—	Incubation: 400 rpm	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Ethanol	20 % v/v	100	98	%	Digital	Continuous	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 60°C, Assay: 60°C	0.25% soluble Chitosan	Reducing sugars	—	Incubation: 400 rpm	Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Ethyl Acetate

10 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Acetonitrile

50 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Acetonitrile

40 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Acetonitrile

30 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Acetonitrile

20 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Acetonitrile

10 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Chloroform

50 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Chloroform

40 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Chloroform

30 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Chloroform

20 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Chloroform

10 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Ethyl Acetate

50 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Ethyl Acetate

40 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Ethyl Acetate

30 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Ethyl Acetate

20 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Ethanol

10 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Acetone

50 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Acetone

40 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Acetone

30 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Acetone

20 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Acetone

10 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Methanol

50 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Methanol

40 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Methanol

30 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Methanol

20 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Methanol

10 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Ethanol

50 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Ethanol

40 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Ethanol

30 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 55°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase

Ethanol

20 % v/v

100

Digital

Continuous

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 60°C

0.25% soluble Chitosan

Reducing sugars

—

Incubation: 400 rpm

Non-incubated control (in %), assay in aqueous phase

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Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Chitosinase (gene ChoK) from Bacillus sp. CK4

Enzyme Description

Sequence

Experimental Data (30 measurements)

Visualization : Stability — Incubation

Structure

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