GH5 endoglucanase from Bacillus sp. G131

Enzyme Description

Species
Bacillus sp. G131 β†—
Extremophile
No but "thermostable" enzyme
EC Number
3.2.1.4 β†—

Sequence

Length: 461 amino acids
ASAASQTPVAVNGQLTLKGTQLVNQKGKAVQLKGISSHGLQWYGDYVNKDSKLWLRDDWGINVFRAAMYTGEGGYIDNPSVKNKVKEAVEAAKELGIYVIIDWHILSDGNPNQNKAKAKEFFNEMSRLYGKTPNVIFEIANEPNGDNVWNNDIKPYAEDILSVIRKNSPKNIVIVGTGTWSQDVNDAADNQLKDGNVMYALHFYAGTHGQSLRDKADYALSKGAPIFVTEWGTSDASGNGGVYLDQSREWLKYLDSKKISWVNWSLCDKQESSAALNSGASKKGGWSQSDlSSSGKFVRENIRSGSNGSSGDSGSDSKGSDQKDQKKDQDKPGQDSGAAANTIAVQYRAGDNNVNGNQIRPQLNIKNNSKKTVSLNRITVRYWYKTNHKGQNFDCDYAQIGCSKLTHKFVQLKKAVNGADTYLEVGFKNGTLAPGASTGEIQIRLHNDNWSNYAQIGDYSF
Nazanin Gholampour-Faroji et al. (2024) β€” Characterization, structural, and evolutionary analysis of an extremophilic GH5 endoglucanase from Bacillus sp. G131: Insights from ancestral sequence reconstruction
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2024.134311 β†—  Β· Stability - Incubation
15 measurements ▼
Database ID
NCBI: XCG98628.1 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number (first 26 residues from GenBank sequence absent from the mature protein)
Protein Source
Purified, bacterium heat-adapted extremophileBacillus sp. G131

Experimental Data (15 measurements)

15 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Dimethyl Sulfoxide (DMSO) 10 % v/v 100.0 65.09 % Direct Continuous ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Methanol 10 % v/v 100.0 61.67 % Direct Continuous ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Ethanol 10 % v/v 100.0 67.86 % Direct Continuous ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Acetone 10 % v/v 100.0 61.97 % Direct Continuous ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Isopropanol 10 % v/v 100.0 70.72 % Direct Continuous ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Dimethyl Sulfoxide (DMSO) 20 % v/v 100.0 54.05 % Direct Continuous ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Methanol 20 % v/v 100.0 48.17 % Direct Continuous ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Ethanol 20 % v/v 100.0 57.2 % Direct Continuous ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Acetone 20 % v/v 100.0 52.35 % Direct Continuous ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Isopropanol 20 % v/v 100.0 49.54 % Direct Continuous ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Dimethyl Sulfoxide (DMSO) 30 % v/v 100.0 44.06 % Direct Continuous ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Methanol 30 % v/v 100.0 37.79 % Direct Continuous ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Ethanol 30 % v/v 100.0 43.84 % Direct Continuous ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Acetone 30 % v/v 100.0 37.83 % Direct Continuous ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Isopropanol 30 % v/v 100.0 39.86 % Direct Continuous ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate Β· Scroll to zoom Β· Right-click drag to pan Β· Powered by Mol*