Ξ±-amylase (Taka-amylase) from Aspergillus oryzae

Enzyme Description

Species
Aspergillus oryzae β†—
Extremophile
No
EC Number
3.2.1.1 β†—

Sequence

Length: 478 amino acids
ATPADWRSQSIYFLLTDRFARTDGSTTATCNTADQKYCGGTWQGIIDKLDYIQGMGFTAIWITPVTAQLPQTTAYGDAYHGYWQQDIYSLNENYGTADDLKALSSALHERGMYLMVDVVANHMGYDGAGSSVDYSVFKPFSSQDYFHPFCFIQNYEDQTQVEDCWLGDNTVSLPDLDTTKDVVKNEWYDWVGSLVSNYSIDGLRIDTVKHVQKDFWPGYNKAAGVYCIGEVLDGDPAYTCPYQNVMDGVLNYPIYYPLLNAFKSTSGSMDDLYNMINTVKSDCPDSTLLGTFVENHDNPRFASYTNDIALAKNVAAFIILNDGIPIIYAGQEQHYAGGNDPANREATWLSGYPTDSELYKLIASANAIRNYAISKDTGFVTYKNWPIYKDDTTIAMRKGTDGSQIVTILSNKGASGDSYTLSLSGAGYTAGQQLTEVIGCTTVTVGSDGNVPVPMAGGLPRVLYPTEKLAGSKICSSS
Khomaini Hasan et al. (2024) β€” Enzymatic Performance of Aspergillus oryzae Ξ±-Amylase in the Presence of Organic Solvents: Activity, Stability, and Bioinformatic Studies
Bioinformatics and Biology Insights  Β· doi:10.1177/11779322241234767 β†—  Β· Activity - Classical
20 measurements ▼
Database ID
PDB: 7TAA β†—
Sequence Annotation
Inferred - sequence of PDB given by authors and used for MD simulations
Protein Source
Commercially purchased

Experimental Data (20 measurements)

20 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent 1-Butanol 25 % v/v 100.0 0.0 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent Diethyl Ether 30 % v/v 100.0 0.0 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent Diethyl Ether 25 % v/v 100.0 0.0 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent Diethyl Ether 20 % v/v 100.0 20.0 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent Diethyl Ether 15 % v/v 100.0 14.88 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent Acetone 30 % v/v 100.0 0.0 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent Acetone 25 % v/v 100.0 0.0 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent Acetone 20 % v/v 100.0 0.0 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent Acetone 15 % v/v 100.0 0.0 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent 1-Butanol 30 % v/v 100.0 0.0 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent Methanol 15 % v/v 100.0 0.0 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent 1-Butanol 20 % v/v 100.0 0.0 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent 1-Butanol 15 % v/v 100.0 0.0 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent Ethanol 30 % v/v 100.0 0.0 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent Ethanol 25 % v/v 100.0 18.48 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent Ethanol 20 % v/v 100.0 29.25 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent Ethanol 15 % v/v 100.0 15.2 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent Methanol 30 % v/v 100.0 0.0 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent Methanol 25 % v/v 100.0 0.0 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent Methanol 20 % v/v 100.0 0.0 % Direct Continuous ? ? 50Β°C 0.002 Starch Reducing sugars β€” β€” Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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