Ξ²-glucosidase (gene RpBgl8) from Reticulitermes perilucifugu

Enzyme Description

Species
Reticulitermes perilucifugus β†—
Extremophile
No
EC Number
3.2.1.21 β†—

Sequence

Length: 476 amino acids
MSALKFPPGFLFGSATSAYQVEGAWNEDGKSDSIWDRLTHDHPEIIKDKSSGDVACNSYHLYKEDVRILKELGVHFYRFSVSWTRILPTGYDNVVNQAGIDYYNNLINELIANGIQPMITMYHFDLPQPLQNLGGWTNPVIANYFEDYARVLYANFGDRVKWWNTINEPKTVALGYSAPFGLAPHVLTPGHGQYLVIHTILLSHARAYRLYEREFKDKQGGKVSIVASCQWIEPTTDSKEEEDAAERARQINLGWELHPIYSADGDYPPVMKQWISKKSKEEGYSRSRLPSFTKEEIELVRGTWDYLALNTYTTFFVRQSDEDSVFLSDTGVVSIPNDKYATASSEWLQVVPWGFRKLLNWIAKEYNNPPVLITENGFSDHGEINDTDRINFYITYLCELLKATKENGCNVIGYTAWSMMDNYEWRSGYTEKFGLFHVDFNDPDRKRTAKKSARVFSQIIKSNAIPVEFLAVETSH
Guotao Mao et al. (2025) β€” Biochemical and Structural Characterization of a Highly Glucose-Tolerant Ξ²-Glucosidase from the Termite Reticulitermes perilucifugus
International Journal of Molecular Sciences  Β· doi:10.3390/ijms26073118 β†—  Β· Activity - Classical
17 measurements ▼
Database ID
UniProt: A0A7G5VX36 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (17 measurements)

17 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 408 nm) in the presence of organic solvent Isopropanol 10 % v/v 100 165 % Digital Continuous 20 mM phosphate buffer 7 37Β°C 5 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 408 nm) in the presence of organic solvent Ethanol 20 % v/v 100 55 % Digital Continuous 20 mM phosphate buffer 7 37Β°C 5 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 408 nm) in the presence of organic solvent Ethanol 15 % v/v 100 96 % Digital Continuous 20 mM phosphate buffer 7 37Β°C 5 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 408 nm) in the presence of organic solvent Ethanol 10 % v/v 100 180 % Digital Continuous 20 mM phosphate buffer 7 37Β°C 5 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 408 nm) in the presence of organic solvent Ethanol 5 % v/v 100 202 % Digital Continuous 20 mM phosphate buffer 7 37Β°C 5 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 408 nm) in the presence of organic solvent Ethanol 2.5 % v/v 100 183 % Digital Continuous 20 mM phosphate buffer 7 37Β°C 5 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 408 nm) in the presence of organic solvent Isopropanol 25 % v/v 100 31 % Digital Continuous 20 mM phosphate buffer 7 37Β°C 5 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 408 nm) in the presence of organic solvent Isopropanol 20 % v/v 100 51 % Digital Continuous 20 mM phosphate buffer 7 37Β°C 5 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 408 nm) in the presence of organic solvent Isopropanol 15 % v/v 100 75 % Digital Continuous 20 mM phosphate buffer 7 37Β°C 5 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 408 nm) in the presence of organic solvent Methanol 2.5 % v/v 100 232 % Digital Continuous 20 mM phosphate buffer 7 37Β°C 5 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 408 nm) in the presence of organic solvent Isopropanol 5 % v/v 100 168 % Digital Continuous 20 mM phosphate buffer 7 37Β°C 5 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 408 nm) in the presence of organic solvent Isopropanol 2.5 % v/v 100 192 % Digital Continuous 20 mM phosphate buffer 7 37Β°C 5 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 408 nm) in the presence of organic solvent Glycerol 5 % v/v 100 130 % Digital Continuous 20 mM phosphate buffer 7 37Β°C 5 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 408 nm) in the presence of organic solvent Glycerol 2.5 % v/v 100 172 % Digital Continuous 20 mM phosphate buffer 7 37Β°C 5 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 408 nm) in the presence of organic solvent 1-Butanol 5 % v/v 100 68 % Digital Continuous 20 mM phosphate buffer 7 37Β°C 5 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 408 nm) in the presence of organic solvent 1-Butanol 2.5 % v/v 100 160 % Digital Continuous 20 mM phosphate buffer 7 37Β°C 5 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 408 nm) in the presence of organic solvent Methanol 5 % v/v 100 102 % Digital Continuous 20 mM phosphate buffer 7 37Β°C 5 mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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