Lipase from Monascus pilosus isolate F15

Enzyme Description

Species
Monascus pilosus isolate F15 β†—
Extremophile
Yes "acidophilic" organism
EC Number
3.1.1.3 β†—

Sequence

Length: 474 amino acids
MDTLAGPNITDKQTILTFAKMTQNDYVEEPGTEDWEDINGPFNYSRSFGWQGDGLRGHIYADKENSTIVISLKGTSPALFDGAGTTTNDKVNDNLYFSCCCGQGGSYLWRQVCDCQKSPYTANLTCIIESMTDENRYYRAAIDLYSNVTELYPNSSVWLTGHSLGGAMTSLLGLTFGLPVVTFEAVPEALPAARLGLPSPPGNDPRLPQKRQYTGSYHFGHTADPVYMGTCNGVNSVCTWGGYALESACHTGQMCVYDTVGDKGWRVGMGTHRIKAVIRDVLEAYDDVPPCAPEEECYDCELWKFFRSNGSESTTTTTTTSTTSTTTSTTLTATRTSTCETPGWWGCLDETTTTTTASMTTTTSTCKTPGWFGCKDPTTTTSATLTPPGTTTPSTTTTTTTSCADPGWFGCRDPTSTVTATSTCETPGYFWGCLDTPTNSPHRITSGPTPPPTITPSGGRDMCTSRAFLGFVCV
Mohamed M.M. Ahmed et al. (2025) β€” Optimization, characterization, and molecular modelling of an acid and organic solvent-tolerant lipase isolated from Monascus pilosus as a potential detergent additive
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2025.144883 β†—  Β· Stability - Incubation
30 measurements ▼
Database ID
UniParc: UPI003D5ACE49 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Purified, fungus Moascus pilosus strain F15 (produced by natural fungal expression using submerged fermentation)

Experimental Data (30 measurements)

30 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 10 % v/v 100.0 78.3 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetic Acid 30 % v/v 100.0 2.5 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetic Acid 20 % v/v 100.0 4.9 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetic Acid 10 % v/v 100.0 7.2 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 30 % v/v 100.0 55.7 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 20 % v/v 100.0 84.8 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10 % v/v 100.0 88.4 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Toluene 30 % v/v 100.0 71.2 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Toluene 20 % v/v 100.0 83.3 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Toluene 10 % v/v 100.0 89.9 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Hexane 30 % v/v 100.0 51.0 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Hexane 20 % v/v 100.0 71.7 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Hexane 10 % v/v 100.0 84.8 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 30 % v/v 100.0 13.1 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 20 % v/v 100.0 50.4 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Chloroform 10 % v/v 100.0 92.0 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 30 % v/v 100.0 3.0 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 20 % v/v 100.0 19.8 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 10 % v/v 100.0 49.8 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 30 % v/v 100.0 19.4 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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