Type I Baeyer–Villiger monooxygenase from Streptomyces sp. G3A

Enzyme Description

Species
Streptomyces sp. G3A β†—
Extremophile
No
EC Number
1.14.13.22 β†—

Sequence

Length: 602 amino acids
MTNPATLSSEELAELRRRYRLERERRVRPDGSRQYLAADAEFGYYADDPYARKVGDRESLRDRVDVAVIGGGFGGILAGARLRQQGIGRVRILEKGGDFGGTWYWNRYPGIHCDIESHVYLPMLDETGYVPEWKYAPGEEIRRHAVGIAEKFDLYADAVFSTAVTSLTWDEISQAWIVTTDRGDKFRATYVVTATGTMSELKLPGIPGIENFKGHTFHTSRWDYAYTGGTPDGGLTGLADKRVGVVGTGATGIQVVPKLAEDAGHLYVFQRTPSTVDVRANRRTIARDVGADHDGWARERRDNFLRITSGEPAEEDLVADQWTASAGLLEKLLPSFRRPADRQAFEAAYEVADAVKMNEIRARVEQIVADADTAEKLKPWYRYACKRPTFSDTYLQAFNRDNVTLVDTADTHGIERMTEHGVVVDGTEYELDCLVFATGFSVGVSGIHSSRLPVHGRNGVQLLHALAERGPRSLHGFTSNGFPNLIQLGATQSASSVNYTHVLDEHAVHAAALVAAAEAKGAVIEPSRQAEDAWLAVMAEDAADHEWFHAECTPGYYNGEGRGRPNAPLAYPHGAFAFHDLLKRWREESMDDVLVVRKASGA
Carolina GonzΓ‘lez et al. (2025) β€” Recombinant Expression and Characterization of a Novel Type I Baeyer–Villiger Monooxygenase from a Streptomyces Strain Isolated from the Rhizosphere of the Atacama Desert Lupinus oreophilus
International Journal of Molecular Sciences  Β· doi:10.3390/ijms26135940 β†—  Β· Activity - Classical
14 measurements ▼
Database ID
UniParc: UPI003C902520 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (14 measurements)

14 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) Acetone 5 % v/v 100.0 94.2 % Digital Continuous 50 mM Tris-HCl buffer 7.5 30Β°C 5 mM Bicyclo(3.2.0)hept-2-en-6-one bicyclo[3.2.0]hept-2-en-6-olide 170 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Acetone 10 % v/v 100.0 91.9 % Digital Continuous 50 mM Tris-HCl buffer 7.5 30Β°C 5 mM Bicyclo(3.2.0)hept-2-en-6-one bicyclo[3.2.0]hept-2-en-6-olide 170 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Acetonitrile 5 % v/v 100.0 91.9 % Digital Continuous 50 mM Tris-HCl buffer 7.5 30Β°C 5 mM Bicyclo(3.2.0)hept-2-en-6-one bicyclo[3.2.0]hept-2-en-6-olide 170 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Acetonitrile 10 % v/v 100.0 89.3 % Digital Continuous 50 mM Tris-HCl buffer 7.5 30Β°C 5 mM Bicyclo(3.2.0)hept-2-en-6-one bicyclo[3.2.0]hept-2-en-6-olide 170 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dichloromethane 5 % v/v 100.0 104.6 % Digital Continuous 50 mM Tris-HCl buffer 7.5 30Β°C 5 mM Bicyclo(3.2.0)hept-2-en-6-one bicyclo[3.2.0]hept-2-en-6-olide 170 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dichloromethane 10 % v/v 100.0 105.5 % Digital Continuous 50 mM Tris-HCl buffer 7.5 30Β°C 5 mM Bicyclo(3.2.0)hept-2-en-6-one bicyclo[3.2.0]hept-2-en-6-olide 170 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5 % v/v 100.0 84.1 % Digital Continuous 50 mM Tris-HCl buffer 7.5 30Β°C 5 mM Bicyclo(3.2.0)hept-2-en-6-one bicyclo[3.2.0]hept-2-en-6-olide 170 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100.0 58.6 % Digital Continuous 50 mM Tris-HCl buffer 7.5 30Β°C 5 mM Bicyclo(3.2.0)hept-2-en-6-one bicyclo[3.2.0]hept-2-en-6-olide 170 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 5 % v/v 100.0 88.7 % Digital Continuous 50 mM Tris-HCl buffer 7.5 30Β°C 5 mM Bicyclo(3.2.0)hept-2-en-6-one bicyclo[3.2.0]hept-2-en-6-olide 170 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 10 % v/v 100.0 73.0 % Digital Continuous 50 mM Tris-HCl buffer 7.5 30Β°C 5 mM Bicyclo(3.2.0)hept-2-en-6-one bicyclo[3.2.0]hept-2-en-6-olide 170 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 5 % v/v 100.0 90.7 % Digital Continuous 50 mM Tris-HCl buffer 7.5 30Β°C 5 mM Bicyclo(3.2.0)hept-2-en-6-one bicyclo[3.2.0]hept-2-en-6-olide 170 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 10 % v/v 100.0 83.2 % Digital Continuous 50 mM Tris-HCl buffer 7.5 30Β°C 5 mM Bicyclo(3.2.0)hept-2-en-6-one bicyclo[3.2.0]hept-2-en-6-olide 170 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Isopropanol 5 % v/v 100.0 77.1 % Digital Continuous 50 mM Tris-HCl buffer 7.5 30Β°C 5 mM Bicyclo(3.2.0)hept-2-en-6-one bicyclo[3.2.0]hept-2-en-6-olide 170 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Isopropanol 10 % v/v 100.0 77.1 % Digital Continuous 50 mM Tris-HCl buffer 7.5 30Β°C 5 mM Bicyclo(3.2.0)hept-2-en-6-one bicyclo[3.2.0]hept-2-en-6-olide 170 Β΅M NADPH β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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