Ξ±-L-rhamnosidase from Dictyoglomus thermophilum

Enzyme Description

Species
Dictyoglomus thermophilum β†—
Extremophile
Yes thermophilic organism
EC Number
3.2.1.40 β†—

Sequence

Length: 921 amino acids
MKSSNIYSPFDLKCEFTTNPLGVDKKNPIFSWKLRHLEKNEKQTAYQVIVSSSLETINDNIGDVWDTGKVLSSEQVIKYEGKELEPCKVYFWKVRWWDSKDQESPFSVVNTFETGLMNEENWKAKWITKKEHKYEVYSPDGAPFGLNYTIAYAPMFRKSFSISKKIKRARVYIAGLGLYELYINGERIGDRVLDPGQTDYKKRVLYTVYDVSKNIRDGKNAIGVILGNGRYVKEYGYDFPKLIIQVLVEYEDDSIEWIVSDESWKTTYGPITLNSLYHGEIYDGRKEIKGWNLPDFDDSTWENAILAEPPGGKLYSEIYPPIRITKTIKPIKMWSPEPGTYVYDFGQNYTGWIKIKVRTNESGKEIRIRHAELTYEDGTLNYSTNRTALATDVYITKGEGYEEYEPRFTYHGFRYVEILGYPGVPTLEDIEGKVVHTAVESNGEFICSNELINKIHHNIIWGQLSNLMSIPTDCPQRDERMGWMGDAQLSAEEAIFNFDMIGFYRKYLNDIRDAQKENGSLSDVIPPYWSIYPGDPAWSTAYITIAWYLYQYYGDKYVLEEHYEGFKKYVEFLKKLAPDYIVSFYKYGDWCQPGTVRPKDNSGELTSTFYFYHDVITLSKIAKLLGKEADYKYYSELADKIKSAFNKKFLKEKAYASIPTELSEENVKALLEKYPEDIKDFLRQQFTILSSLGMFTSQTLNTLPLYLNLVPEDKVQDVLKTLLEDIIIRHDYHLDTGIVATRYIFDVLTSYGYDEVAYKIVNQKTYPSFGYMIEEGATTLWERWEKLTSTGMNSHNHIMFGSVDAWFYRVIAGVRVGEPGWNKIIFEPHPVGDLKYAKARLNTIKGEVEINWQKTENIFSMRISVPVNSEGEVHVPKLFERFVVKEGDNIIYEKKGDLEENEKYIVIRVGSGSYNFYMEKI
Jinyue Hu et al. (2025) β€” An Organic Solvent-Tolerant Ξ±-L-Rhamnosidase from Dictyoglomus thermophilum and Its Application in Production of Icariside I from Icariin
Molecules  Β· doi:10.3390/molecules30132847 β†—  Β· Activity - Classical Stability - Incubation Activity + Stability - Conversion
57 measurements ▼
Database ID
UniProt: B5YC64 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (57 measurements)

57 measurements β€” page 1 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 20 % v/v 100 144 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 10 % v/v 100 96 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 40 % v/v 100 113 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20 % v/v 100 121 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100 188 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetonitrile 40 % v/v 100 72 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetonitrile 20 % v/v 100 90 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetonitrile 10 % v/v 100 118 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 40 % v/v 100 97 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 20 % v/v 100 161 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 10 % v/v 100 102 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 40 % v/v 100 102 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 55Β°C) in the presence of organic solvent, measured by HPLC (icariin concentration) Dimethyl Sulfoxide (DMSO) 5 % v/v 50 75 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM Icariin icariside I β€” 180 rpm Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (1 hour at 55Β°C) in the presence of organic solvent, measured by HPLC (icariin concentration) Dimethyl Sulfoxide (DMSO) 5 % v/v 28 36 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM Icariin icariside I β€” 180 rpm Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (4 hours at 55Β°C) in the presence of organic solvent, measured by HPLC (icariin concentration) Dimethyl Sulfoxide (DMSO) 5 % v/v 62 90 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM Icariin icariside I β€” 180 rpm Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (6 hours at 55Β°C) in the presence of organic solvent, measured by HPLC (icariin concentration) Dimethyl Sulfoxide (DMSO) 5 % v/v 63 91 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM Icariin icariside I β€” 180 rpm Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (8 hours at 55Β°C) in the presence of organic solvent, measured by HPLC (icariin concentration) Dimethyl Sulfoxide (DMSO) 5 % v/v 66 91 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM Icariin icariside I β€” 180 rpm Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (1 hour at 55Β°C) in the presence of organic solvent, measured by HPLC (icariin concentration) Dimethyl Sulfoxide (DMSO) 10 % v/v 28 50 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM Icariin icariside I β€” 180 rpm Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 55Β°C) in the presence of organic solvent, measured by HPLC (icariin concentration) Dimethyl Sulfoxide (DMSO) 10 % v/v 50 90 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM Icariin icariside I β€” 180 rpm Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (4 hours at 55Β°C) in the presence of organic solvent, measured by HPLC (icariin concentration) Dimethyl Sulfoxide (DMSO) 10 % v/v 62 96 % Digital Continuous 100 mM sodium acetate buffer 6 55Β°C 3 mM Icariin icariside I β€” 180 rpm Classical aqueous control (in %)
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity + Stability β€” Conversion

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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