Lipase from Kurtzmanomyces sp. I-11

Enzyme Description

Species
Kurtzmanomyces sp. I-11 β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 432 amino acids
AALPDPNEDPFYSTPSNIETFANGQIIQSRKVPTDIGNSNNAASYQLSYRTTNTQEDAVANVATIWIPAKPSSPPRIFTYQVYEDSTQLDCAPSYSYLTGYDQPNKATAVLDTPIVISWALQQGYYVVSADHEGARSAFIAGYEEGMAALDGIRALRNYAKLPQDSAVGAYGYSGGAHATVWATSLAAAYAPEINFIGAAHGGTPVSAKDTFTFINGGFFAGFAIAGVSGLANAHPDMEAFIQPRLNAEGVKTLKQIRSRGFCLPEVVTTYPFKNVFALVNDTNLLTEQPISGILQQETLVQSEASYAVPVPKFPRFLWHAALDEIVPYVPVTEYVKEQCAKGANINFNTYPIAEHLTAEIFGLVPGLWFLSQAYEGKAPAVQCGTALPAAPSAQQVLGNDLANQLSSLNGKQSPFGKPFGPISPTSLDKLL
Koji Kakugawa et al. (2002) β€” Purification and Characterization of a Lipase from the Glycolipid-Producing Yeast Kurtzmanomyces sp. I-11
Bioscience, Biotechnology, and Biochemistry  Β· doi:10.1271/bbb.66.978 β†—  Β· Stability - Incubation
56 measurements ▼
Database ID
UniProt: Q8NIP2 β†—
Sequence Annotation
Inferred - from protein name (first 26 residues from UniProt sequence absent from the mature protein)
Protein Source
Purified, fungus Kurtzmanomyces sp. I-11

Experimental Data (56 measurements)

56 measurements β€” page 2 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethyl Acetate 20 % v/v 100 112 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethyl Acetate 40 % v/v 100 104 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethyl Acetate 60 % v/v 100 114 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethyl Acetate 80 % v/v 100 116 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 20 % v/v 100 101 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 40 % v/v 100 102 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 60 % v/v 100 86 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 80 % v/v 100 100 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase 1-Butanol 60 % v/v 100 111 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 40 % v/v 100 98 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 60 % v/v 100 66 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 80 % v/v 100 10 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 20 % v/v 100 107 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 40 % v/v 100 93 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 60 % v/v 100 99 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 80 % v/v 100 92 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 20 % v/v 100 99 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 40 % v/v 100 102 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 60 % v/v 100 105 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 80 % v/v 100 57 % Digital Continuous 50 mM acetate buffer 5.6 Incubation: 30Β°C, Assay: 37Β°C 2.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
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Visualization : Stability β€” Incubation

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