Lipase (gene StSGNH1) from Sphaerobacter thermophilus

Enzyme Description

Species
Sphaerobacter thermophilus β†—
Extremophile
Yes thermophilic organism
EC Number
3.1.1.3 β†—

Sequence

Length: 373 amino acids
HHHHHHSSGVDLGTENLYFQSNAMRRTRSALMVALLIATALAPLAARSTPRVAAQPALPEQVYLALGDSIAAGLVTSLPRVRGYPWLVRDLMEKHFTSEGAPAVTLINRAVPGETTTSFLAGDQLREARADIEAARARGAEVRAVTVTLGGNDILRLAGSDEAERQAGLVQFRSTFPAALAAIREALGEMPADIVVTTYYDLSEGNPAEEGSDAWWVAQFNEVIRQSAAAEGARVADVEPVFRGNIREWTWYPSDIHANNAGHAEIARLVWQALGYDQEPPTVTIERPAAGPLGRRTPTVRVRADDAVGVTRVELLVDGTYVQDLRYIPSQGAYLGVWDARDWSDASATLTVRATDLAGNQASAEVEVALPGS
Kelsey Minium et al. (2025) β€” Characterization of a Highly Solvent-Tolerant SGNH Hydrolase Superfamily Lipolytic Enzyme from Sphaerobacter thermophilus
Biochemistry  Β· doi:10.1021/acs.biochem.5c00057 β†—  Β· Activity - Classical
19 measurements ▼
Database ID
β€”
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (19 measurements)

19 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent 1-Propanol 50 % v/v 100 101 % Digital Continuous 50 mM phosphate buffer, 1% Triton-X-100 8 25Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetonitrile 50 % v/v 100 89 % Digital Continuous 50 mM phosphate buffer, 1% Triton-X-100 8 25Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetonitrile 30 % v/v 100 179 % Digital Continuous 50 mM phosphate buffer, 1% Triton-X-100 8 25Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetonitrile 10 % v/v 100 108 % Digital Continuous 50 mM phosphate buffer, 1% Triton-X-100 8 25Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetone 50 % v/v 100 10 % Digital Continuous 50 mM phosphate buffer, 1% Triton-X-100 8 25Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetone 30 % v/v 100 58 % Digital Continuous 50 mM phosphate buffer, 1% Triton-X-100 8 25Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetone 10 % v/v 100 121 % Digital Continuous 50 mM phosphate buffer, 1% Triton-X-100 8 25Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Isopropanol 50 % v/v 100 31 % Digital Continuous 50 mM phosphate buffer, 1% Triton-X-100 8 25Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Isopropanol 30 % v/v 100 188 % Digital Continuous 50 mM phosphate buffer, 1% Triton-X-100 8 25Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Isopropanol 10 % v/v 100 138 % Digital Continuous 50 mM phosphate buffer, 1% Triton-X-100 8 25Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 10 % v/v 100 198 % Digital Continuous 50 mM phosphate buffer, 1% Triton-X-100 8 25Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent 1-Propanol 30 % v/v 100 268 % Digital Continuous 50 mM phosphate buffer, 1% Triton-X-100 8 25Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent 1-Propanol 10 % v/v 100 169 % Digital Continuous 50 mM phosphate buffer, 1% Triton-X-100 8 25Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 50 % v/v 100 0 % Digital Continuous 50 mM phosphate buffer, 1% Triton-X-100 8 25Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 30 % v/v 100 0 % Digital Continuous 50 mM phosphate buffer, 1% Triton-X-100 8 25Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 10 % v/v 100 95 % Digital Continuous 50 mM phosphate buffer, 1% Triton-X-100 8 25Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 70 % v/v 100 63 % Digital Continuous 50 mM phosphate buffer, 1% Triton-X-100 8 25Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 50 % v/v 100 462 % Digital Continuous 50 mM phosphate buffer, 1% Triton-X-100 8 25Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 30 % v/v 100 377 % Digital Continuous 50 mM phosphate buffer, 1% Triton-X-100 8 25Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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