Multicopper oxidase (gene BmLMCO) from Bacillus mojavensis TH309

Enzyme Description

Species
Bacillus mojavensis TH309 β†—
Extremophile
No
EC Number
1.10.3.2 β†—

Sequence

Length: 466 amino acids
MTLEKFADALPIPDTLKPVQQSKDSTYYAVTMEECYHQLHRDLPPTRLWGYNGLFPGPTGEAFRNENVYVKWMFNLPSIHFLPIGHTIFHSDSQHAEVEVWAHDHAMALTRLNVYAGLIGAYAIHDPKEKRLKLPSGEYDVPLLITARTINEDGSLFYPSGPENPSHHCASSIVSFWRRQQFSSTEHAYMSRTRDYAPRHHASIPRHILASLGYAVHYGSIGRTLRIRQAGGLLPRSVDLNSASIAPCAERFDILIDWFAAFEGQSIIIANSEGAGGDVNPETDANIMQFRVTKPLAQKDESRKGPKYLAGSYPSVQHRAIQNLRTLKLAGTQDQHHYGTRPVLLLHNKRWHHDPVTEAGKAGSTEVWSIINPTRGTHPIHLHLVSFRAGLDRRPFDTARSEERGELFYTGPAVPFTPHSEKGFWKDTVIDSHGGEVLRIAVTFGPYTSRYAVTFGPYTSRYDPHK
Ali Osman AdigΓΌzel et al. (2023) β€” Heterologous expression, purification, and characterization of thermo- and alkali-tolerant laccase-like multicopper oxidase from Bacillus mojavensis TH309 and determination of its antibiotic removal potential
Archives of Microbiology  Β· doi:10.1007/s00203-023-03626-5 β†—  Β· Activity - Classical
14 measurements ▼
Database ID
β€”
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (14 measurements)

14 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical reaction products absorbance measurement, 470 nm) in the presence of organic solvent Ethanol 10 % v/v 100.0 62.8 % Digital Continuous 50 mM Tris-HCl buffer 8 80Β°C 2.96 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 0.27 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical reaction products absorbance measurement, 470 nm) in the presence of organic solvent Ethanol 30 % v/v 100.0 81.4 % Digital Continuous 50 mM Tris-HCl buffer 8 80Β°C 2.96 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 0.27 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical reaction products absorbance measurement, 470 nm) in the presence of organic solvent Acetone 10 % v/v 100.0 80.0 % Digital Continuous 50 mM Tris-HCl buffer 8 80Β°C 2.96 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 0.27 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical reaction products absorbance measurement, 470 nm) in the presence of organic solvent Acetone 30 % v/v 100.0 115.8 % Digital Continuous 50 mM Tris-HCl buffer 8 80Β°C 2.96 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 0.27 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical reaction products absorbance measurement, 470 nm) in the presence of organic solvent Methanol 10 % v/v 100.0 86.4 % Digital Continuous 50 mM Tris-HCl buffer 8 80Β°C 2.96 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 0.27 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical reaction products absorbance measurement, 470 nm) in the presence of organic solvent Methanol 30 % v/v 100.0 86.1 % Digital Continuous 50 mM Tris-HCl buffer 8 80Β°C 2.96 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 0.27 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical reaction products absorbance measurement, 470 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100.0 78.6 % Digital Continuous 50 mM Tris-HCl buffer 8 80Β°C 2.96 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 0.27 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical reaction products absorbance measurement, 470 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30 % v/v 100.0 92.2 % Digital Continuous 50 mM Tris-HCl buffer 8 80Β°C 2.96 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 0.27 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical reaction products absorbance measurement, 470 nm) in the presence of organic solvent Chloroform 10 % v/v 100.0 98.6 % Digital Continuous 50 mM Tris-HCl buffer 8 80Β°C 2.96 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 0.27 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical reaction products absorbance measurement, 470 nm) in the presence of organic solvent Chloroform 30 % v/v 100.0 124.2 % Digital Continuous 50 mM Tris-HCl buffer 8 80Β°C 2.96 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 0.27 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical reaction products absorbance measurement, 470 nm) in the presence of organic solvent 1-Butanol 10 % v/v 100.0 75.6 % Digital Continuous 50 mM Tris-HCl buffer 8 80Β°C 2.96 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 0.27 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical reaction products absorbance measurement, 470 nm) in the presence of organic solvent 1-Butanol 30 % v/v 100.0 88.3 % Digital Continuous 50 mM Tris-HCl buffer 8 80Β°C 2.96 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 0.27 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical reaction products absorbance measurement, 470 nm) in the presence of organic solvent Isopropanol 10 % v/v 100.0 99.7 % Digital Continuous 50 mM Tris-HCl buffer 8 80Β°C 2.96 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 0.27 mM CuCl2 β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2.6-dimethoxyphenoxy radical reaction products absorbance measurement, 470 nm) in the presence of organic solvent Isopropanol 30 % v/v 100.0 108.0 % Digital Continuous 50 mM Tris-HCl buffer 8 80Β°C 2.96 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical 0.27 mM CuCl2 β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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