Phosphite dehydrogenase (gene Ct-PtxD) from Cyanothece sp. ATCC 51142
Enzyme Description
Sequence
MNQKPKVVITHWVHPEIIDYLTPHCELILNQTKETLTREEVINRSKDAQGLMVFMPDYIDVNFLEACPQLKVISGALRGYDNFDVEACTKRNIWFTIVPDLLAAPTAELTIGLLLILARRMVEGDRLIRSGNFQGWKPQLYSTGLLNKTLGIIGMGKLGKALTKRLMGFDMTLLYHDKITLTSQQERDWKITKTSLEELLTKSDYVVLMVPLVPDTYHLINENSLKMMKPNSFLINPCRGSIVDETAVATAIKSGHLAGYAADVFEMEDWAIANRPQSINQTLLTDINHTFFTPHLGSAVNEVRRDIALEAAKNIIEVLSENRPQGAVNGIV
Gamal Nasser Abdel-Hady et al. (2023)
β A novel salt- and organic solvent-tolerant phosphite dehydrogenase from Cyanothece sp. ATCC 51142
Frontiers in Bioengineering and Biotechnology
Β· doi:10.3389/fbioe.2023.1255582 β
Β· Activity - Classical Stability - Incubation
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Database ID
NCBI: WP_009544749.1 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 pLysS
Experimental Data (40 measurements)
40 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Extraction method | Data type | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 20 % v/v | 100.0 | 159.3 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 10 % v/v | 100.0 | 125.2 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 50 % v/v | 100.0 | 7.6 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 40 % v/v | 100.0 | 55.8 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 30 % v/v | 100.0 | 94.3 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20 % v/v | 100.0 | 113.8 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10 % v/v | 100.0 | 112.2 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Ethanol | 50 % v/v | 100.0 | 5.4 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Ethanol | 40 % v/v | 100.0 | 1.1 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Ethanol | 30 % v/v | 100.0 | 28.2 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Ethanol | 20 % v/v | 100.0 | 168.0 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Methanol | 50 % v/v | 100.0 | 1.1 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Methanol | 40 % v/v | 100.0 | 3.2 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Methanol | 30 % v/v | 100.0 | 186.4 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Methanol | 20 % v/v | 100.0 | 166.9 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Methanol | 10 % v/v | 100.0 | 139.3 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 50 % v/v | 100.0 | 3.8 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 40 % v/v | 100.0 | 44.4 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 30 % v/v | 100.0 | 158.8 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Ethanol | 10 % v/v | 100.0 | 147.4 | % | Digital | Continuous | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.